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PMID: 218215 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Integration and transcription of mouse mammary tumor virus DNA in rat hepatoma cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 2 ·1979-02-00 ·Pages 665-9

Ringold GM, Shank PR, Varmus HE, Ring J, Yamamoto KR

Abstract

Rat hepatoma cells infected with mouse mammary tumor virus (MMTV) acquire viral DNA that becomes covalently linked to the cell DNA. Using restriction endonucleases and the DNA transfer procedure of Southern [Southern , E.M. (1975) J. Mol. Biol. 98, 503--517], we have studied the sites in cellular DNA into which MMTV DNA inserts. These experiments indicate that: (i) there are many sites in cell DNA into which MMTV DNA integrates; (ii) the junctions between viral and cellular DNA occur within a limited portion of the viral genome, (iii) clones that contain MMTV DNA do not necessarily produce viral RNA; and (iv) the extent of transcription and glucocorticoid responsiveness of MMTV proviruses may be dependent on the site(s) in cell DNA in which the viral DNA resides.

MeSH Terms
Animals Base Sequence Cell Line Chromosome Mapping DNA, Viral/genetics Genes, Viral Genetic Linkage Liver Neoplasms, Experimental/genetics Mammary Tumor Virus, Mouse/genetics RNA, Viral/genetics Rats Recombination, Genetic
Chemicals
DNA, Viral RNA, Viral
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Ringold G M
Shank P R
Varmus H E
Ring J
Yamamoto K R
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26 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.76.2.665
Published
1979-02-00
Pages
665-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC383010
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.76.2.665 PMC Full Text View journal details
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