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PMID: 2182197 Published · ppublish English Journal Article

Cloning and sequencing of URA10, a second gene encoding orotate phosphoribosyl transferase in Saccharomyces cerevisiae.

Current genetics ·Vol. 17 ·No. 2 ·1990-02-00 ·Pages 105-11

de Montigny J, Kern L, Hubert JC, Lacroute F

Abstract

Orotate phosphoribosyl transferase (OP-RTase) catalyses the transformation of orotate to OMP in the pyrimidine pathway. In the yeast Saccharomyces cerevisiae, the URA5 gene is known to encode this enzyme activity. In this paper we present the cloning and sequencing of a yeast gene, named URA10, encoding a second OPRTase enzyme. Comparison of the predicted amino acid sequences between URA5 and URA10 genes shows more than 75% similarity. These sequences have also been compared to those of Escherichia coli, Podospora anserina, Sordaria macrospora and Dictyostelium discoideum. Remarkable similarities in the primary structure of these proteins have been found. Gene disruption experiments revealed that URA10 gene expression is responsible for the leaky phenotype of a ura5 mutant. Assays of OPRTase activity in extracts from ura5 and ura10 mutants indicate that the URA10 product contributes only 20% of the total activity found in wild type cells.

MeSH Terms
Amino Acid Sequence Base Sequence Blotting, Southern Cloning, Molecular Genes, Fungal Molecular Sequence Data Mutation Orotate Phosphoribosyltransferase/genetics,metabolism Pentosyltransferases/genetics Phenotype Pyrimidines/biosynthesis Restriction Mapping Saccharomyces cerevisiae/enzymology,genetics Sequence Homology, Nucleic Acid
Chemicals
Pyrimidines Pentosyltransferases Orotate Phosphoribosyltransferase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
de Montigny J
Laboratoire de génétique physiologique, I.B.M.C. du C.N.R.S., Strasbourg, France.
Kern L
Hubert J C
Lacroute F
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Article Info
Journal
Current genetics
Abbr.
Curr Genet
ISSN
0172-8083
DOI
10.1007/BF00312853
Published
1990-02-00
Pages
105-11
Language
English
Region
United States
NLM ID
8004904
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00312853 View journal details
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