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PMID: 2185468 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Revision of the oligosaccharide structures of yeast carboxypeptidase Y.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 9 ·1990-05-00 ·Pages 3368-72

Ballou L, Hernandez LM, Alvarado E, Ballou CE

Abstract

The N-linked oligosaccharides from baker's yeast carboxypeptidase Y were analyzed by 1H NMR and specific mannosidase digestion and found to be identical to those from the Saccharomyces cerevisiae mnn9 mutant bulk mannoprotein. The results support the view that the mnn mutants make oligosaccharides that are a true reflection of the normal biosynthetic pathway and confirm that a recently revised yeast oligosaccharide structure is applicable to wild-type mannoproteins.

MeSH Terms
Carbohydrate Conformation Carbohydrate Sequence Carboxypeptidases/genetics Cathepsin A Chromatography, High Pressure Liquid Glycoproteins/genetics Magnetic Resonance Spectroscopy Mannose/analysis Molecular Sequence Data Mutation Oligosaccharides/isolation & purification Phosphorylation Saccharomyces cerevisiae/enzymology,genetics Saccharomyces cerevisiae Proteins
Chemicals
Glycoproteins Oligosaccharides Saccharomyces cerevisiae Proteins Carboxypeptidases Cathepsin A PRC1 protein, S cerevisiae serine carboxypeptidase Mannose
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Ballou L
Department of Biochemistry, University of California, Berkeley 94720.
Hernandez L M
Alvarado E
Ballou C E
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20 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.87.9.3368
Published
1990-05-00
Pages
3368-72
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC53901
Subset
IM
Grants
NIAID NIH HHS · AI-12522 · United States
External Links
PubMed source DOI: 10.1073/pnas.87.9.3368 PMC Full Text View journal details
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