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PMID: 2185472 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Cloning and expression of a mammalian peptide chain release factor with sequence similarity to tryptophanyl-tRNA synthetases.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 9 ·1990-05-00 ·Pages 3508-12

Lee CC, Craigen WJ, Muzny DM, Harlow E, Caskey CT

Abstract

The termination of protein synthesis is encoded by in-frame nonsense (stop) codons. Most organisms use three nonsense codons: UGA, UAG, and UAA. In contrast to sense codons, which are decoded by specific tRNAs, nonsense codons are decoded by proteins called release factors (RFs). Here we report the cloning of a mammalian RF cDNA by the use of monoclonal antibodies specific for rabbit RF. Functional studies showed that, when expressed in Escherichia coli, the protein encoded by this cDNA has in vitro biochemical characteristics similar to those of previously characterized mammalian RFs. DNA sequencing of this eukaryotic RF cDNA revealed a remarkable sequence similarity to bacterial and mitochondrial tryptophanyl-tRNA synthetases, with the greatest similarity confined to the synthetase active site, and no obvious similarity to bacterial RFs.

MeSH Terms
Amino Acid Sequence Amino Acyl-tRNA Synthetases/genetics Animals Base Sequence Binding Sites Cell Line Cloning, Molecular DNA/genetics Escherichia coli/genetics Gene Library Liver/metabolism Molecular Sequence Data Peptide Termination Factors/genetics Rabbits Sequence Homology, Nucleic Acid Transfection Tryptophan-tRNA Ligase/genetics
Chemicals
Peptide Termination Factors peptide chain release factor, mammalian DNA Amino Acyl-tRNA Synthetases Tryptophan-tRNA Ligase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Lee C C
Institute for Molecular Genetics, Baylor College of Medicine, Houston, TX 77030.
Craigen W J
Muzny D M
Harlow E
Caskey C T
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34 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.87.9.3508
Published
1990-05-00
Pages
3508-12
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC53930
Subset
IM
Grants
NIGMS NIH HHS · GM34438 · United States
Databases
GENBANK
M33460
External Links
PubMed source DOI: 10.1073/pnas.87.9.3508 PMC Full Text View journal details
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