The chloroplast is a complex organelle which carries out a wide range of metabolic processes such as light capture and the biosynthesis of carbohydrates, fatty acid and amino acids. This organelle consists of three separate membrane systems which enclose three distinct soluble phases. Most of the chloroplast proteins are imported from the cytosol and directed into the six different compartments. This import and intraorganellar sorting process makes the chloroplast an interesting and promising system for the analysis of how proteins interact with and are translocated across biological membranes.
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