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PMID: 2188961 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Further electron microscopic studies on the expression of Escherichia coli group II capsules.

Journal of bacteriology ·Vol. 172 ·No. 6 ·1990-06-00 ·Pages 3469-72

Kröncke KD, Golecki JR, Jann K

Abstract

The de novo expression of Escherichia coli K1, K5, and K12 capsules was analyzed with immunoelectron microscopy in temperature upshift experiments, with upshift from 18 degrees C (capsule restrictive) to 37 degrees C (capsule permissive). Newly produced capsular polysaccharides appeared at the cell surface atop membrane adhesion sites (Bayer's junctions). After plasmolysis of the bacteria at an early expression stage, the capsular polysaccharides were labeled at discrete sites in the periplasm by the immunogold technique. After temperature upshift in the presence of carbonyl cyanide m-chlorophenylhydrazone (CCCP) or chloramphenicol, the polysaccharides were labeled in the cytoplasm.

MeSH Terms
Carbonyl Cyanide m-Chlorophenyl Hydrazone/pharmacology Chloramphenicol/pharmacology Escherichia coli/analysis,ultrastructure Microscopy, Electron Polysaccharides, Bacterial/analysis Temperature
Chemicals
Polysaccharides, Bacterial Carbonyl Cyanide m-Chlorophenyl Hydrazone Chloramphenicol
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kröncke K D
Max-Planck-Institut für Immunbiologie, Universität Freiburg, Federal Republic of Germany.
Golecki J R
Jann K
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.172.6.3469-3472.1990
Published
1990-06-00
Pages
3469-72
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC209159
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.172.6.3469-3472.1990 PMC Full Text View journal details
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