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PMID: 2191948 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Cloning and characterization of the iron-sulfur subunit gene of succinate dehydrogenase from Saccharomyces cerevisiae.

The Journal of biological chemistry ·Vol. 265 ·No. 18 ·1990-06-25 ·Pages 10419-23

Lombardo A, Carine K, Scheffler IE

Abstract

We describe the cloning and characterization of the complete gene for the iron-sulfur protein subunit of succinate dehydrogenase (EC 1.3.99.1) from Saccharomyces cerevisiae. The promoter and coding sequence have been cloned into an Escherichia coli-yeast shuttle vector. The cloned gene complements the defect in a succinate dehydrogenase-deficient yeast mutant isolated by us, and gene expression is fully responsive to induction by glucose deprivation, indicating that the promoter is intact.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Blotting, Northern Cattle Cloning, Molecular DNA Probes Gene Expression Genes, Fungal Genetic Vectors Iron-Sulfur Proteins/genetics Macromolecular Substances Metalloproteins/genetics Molecular Sequence Data Nucleic Acid Hybridization Restriction Mapping Saccharomyces cerevisiae/enzymology,genetics Sequence Homology, Nucleic Acid Succinate Dehydrogenase/genetics
Chemicals
DNA Probes Iron-Sulfur Proteins Macromolecular Substances Metalloproteins Succinate Dehydrogenase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lombardo A
Department of Biology, University of California, San Diego, La Jolla 92093.
Carine K
Scheffler I E
Article Info
Journal
The Journal of biological chemistry
Abbr.
J Biol Chem
ISSN
0021-9258
Published
1990-06-25
Pages
10419-23
Language
English
Region
United States
NLM ID
2985121R
Subset
IM
Grants
NIGMS NIH HHS · GM33752 · United States
Databases
GENBANK
J05487
External Links
PubMed source View journal details
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