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PMID: 2193021 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S. Review

Terminally differentiating epithelial tissues in primary explant culture: a model of growth and development.

In vitro cellular & developmental biology : journal of the Tissue Culture Association ·Vol. 26 ·No. 6 ·1990-06-00 ·Pages 543-53

Merchant DJ

Abstract

Many epithelial tissues are characterized by the presence of basal cells which serve the dual roles of self-renewal and of progression through terminal differentiation, to a functional state. Such tissues, when grown in vitro as primary explants, exhibit a characteristic pattern of outgrowth and development which includes both renewal and efforts toward normal differentiation. The degree of differentiation achieved depends upon conditions of culture and may be modulated in a variety of ways. The human prostate constitutes such a system and offers numerous possibilities for investigating basic control mechanisms in growth and development. Information on a variety of epithelial tissues is reviewed and experimental results using human prostate tissue are presented.

MeSH Terms
Cell Differentiation Cells, Cultured Culture Techniques/methods Epithelial Cells Growth Humans Models, Biological
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Merchant D J
Department of Microbiology and Immunology, Eastern Virginia Medical School, Norfolk 23501.
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Article Info
Journal
In vitro cellular & developmental biology : journal of the Tissue Culture Association
Abbr.
In Vitro Cell Dev Biol
ISSN
0883-8364
DOI
10.1007/BF02624202
Published
1990-06-00
Pages
543-53
Language
English
Region
United States
NLM ID
8506951
Subset
IM
Grants
NCI NIH HHS · R26 CA 2365 · United States
External Links
PubMed source DOI: 10.1007/BF02624202
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