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PMID: 2196453 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Sequencing of Saccharomyces telomeres cloned using T4 DNA polymerase reveals two domains.

Molecular and cellular biology ·Vol. 10 ·No. 8 ·1990-08-00 ·Pages 4415-9

Wang SS, Zakian VA

Abstract

By using T4 DNA polymerase rather than S1 or Bal31 nuclease to clone yeast telomeres, very little telomeric DNA is lost. These clones were used to determine the DNA sequence of virtually the entire telomeric tract. Our results demonstrated that a slightly modified version, C2-3A(CA)1-6, of the consensus derived from sequence analysis of more-internal regions (J. Shampay, J. W. Szostak, and E. H. Blackburn, Nature [London] 310:154-157, 1984) extends to the very end of the chromosome. The sequence analysis also suggests that yeast telomeres consist of two domains: the proximal 120 to 150 base pairs, which appear to be protected from processes such as recombination, degradation, and elongation, and the distal portion of the telomere, which is more susceptible to these events.

MeSH Terms
Base Sequence Chromosomes, Fungal Cloning, Molecular/methods DNA, Fungal/genetics DNA-Directed DNA Polymerase/metabolism Escherichia coli/genetics Molecular Sequence Data Restriction Mapping Saccharomyces cerevisiae/genetics T-Phages/enzymology
Chemicals
DNA, Fungal DNA-Directed DNA Polymerase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wang S S
Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.
Zakian V A
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29 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.10.8.4415-4419.1990
Published
1990-08-00
Pages
4415-9
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC361005
Subset
IM
Grants
NIGMS NIH HHS · GM26938 · United States
Databases
GENBANK
M34310, M34311, M34312, M34313
External Links
PubMed source DOI: 10.1128/mcb.10.8.4415-4419.1990 PMC Full Text View journal details
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