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PMID: 21965664 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

hSWS1·SWSAP1 is an evolutionarily conserved complex required for efficient homologous recombination repair.

The Journal of biological chemistry ·Vol. 286 ·No. 48 ·2011-12-02 ·Pages 41758-41766

Liu T, Wan L, Wu Y, Chen J, Huang J

Abstract

The Shu complex in yeast plays an important role in the homologous recombination pathway, which is critical for the maintenance of genomic integrity. The identification of human SWS1 (hSWS1) as the homolog of budding yeast Shu2 implicated that the Shu complex is evolutionarily conserved. However, the human counterparts of other components in this complex have not yet been identified and characterized. Here we describe the characterization of a novel human component of this complex, SWSAP1 (hSWS1-associated protein 1)/C19orf39. We show that hSWS1 and SWSAP1 form a stable complex in vivo and in vitro. hSWS1 and SWSAP1 are mutually interdependent for their stability. We further demonstrate that the purified hSWS1·SWSAP1 complex possesses single-stranded DNA-binding activity and DNA-stimulated ATPase activity. Moreover, SWSAP1 interacts with RAD51 and RAD51 paralogs, and depletion of SWSAP1 causes defects in homologous recombination repair. Thus, our results suggest that the human Shu complex (hSWS1·SWSAP1) has an evolutionarily conserved function in homologous recombination.

MeSH Terms
Calcium-Binding Proteins/genetics,metabolism DNA, Single-Stranded/genetics,metabolism DNA-Binding Proteins/genetics,metabolism Evolution, Molecular HeLa Cells Humans Multiprotein Complexes/genetics,metabolism Rad51 Recombinase/genetics,metabolism Recombinational DNA Repair/physiology Saccharomyces cerevisiae/genetics,metabolism
Chemicals
Calcium-Binding Proteins DNA, Single-Stranded DNA-Binding Proteins EFHD2 protein, human Multiprotein Complexes RAD51 protein, human Rad51 Recombinase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Liu Ting
Life Sciences Institute, Zhejiang University, Hangzhou, Zhejiang 310058, China.
Wan Li
Life Sciences Institute, Zhejiang University, Hangzhou, Zhejiang 310058, China.
Wu Yue
Life Sciences Institute, Zhejiang University, Hangzhou, Zhejiang 310058, China.
Chen Junjie
Department of Experimental Radiation Oncology, The University of Texas M.D. Anderson Cancer Center, Houston, Texas 77030. Electronic address: [email protected].
Huang Jun
Life Sciences Institute, Zhejiang University, Hangzhou, Zhejiang 310058, China. Electronic address: [email protected].
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Article Info
Journal
The Journal of biological chemistry
Abbr.
J Biol Chem
ISSN
1083-351X
DOI
10.1074/jbc.M111.271080
Email
[email protected]
[email protected]
Published
2011-12-02
Epub
2011-00-29
Pages
41758-41766
Language
English
Region
United States
NLM ID
2985121R
PMCID
PMC3308884
Subset
IM
Grants
NCI NIH HHS · R01 CA089239 · United States
External Links
PubMed source DOI: 10.1074/jbc.M111.271080 PMC Full Text View journal details
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