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PMID: 21989496 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Sperm transcriptome profiling in oligozoospermia.

Journal of assisted reproduction and genetics ·Vol. 29 ·No. 1 ·2012-01-00 ·Pages 3-10

Montjean D, De La Grange P, Gentien D, Rapinat A, Belloc S, Cohen-Bacrie P, Menezo Y, Benkhalifa M

Abstract

Investigate in what extent sperm transcriptome of infertile men is different from that of fertile individuals. Semen samples were collected for determination of sperm parameters as well as for RNA isolation. Gene expression profile was investigated in spermatozoa of 8 infertile and 3 fertile men by microarray analysis using the Affymetrix Chip HG-U133 Plus 2.0. We observed up to 33-fold reduction expression of genes involved in spermatogenesis and sperm motility. Furthermore, there is an important decrease in expression of genes involved in DNA repair as well as oxidative stress regulation. In this study, we also show a striking drop in expression of histone modification genes. We found that transcription profile in germ cells of men with idiopathic infertility is different from that of fertile individuals. Interestingly, about 15% of the regulated genes (Eddy Rev Reprod 4:23-30, 1999) play a role in spermatogenesis.

MeSH Terms
DNA Repair/genetics Gene Expression Regulation Humans Infertility, Male/genetics Male Oligonucleotide Array Sequence Analysis/methods Oligospermia/genetics,pathology Oxidative Stress/genetics Sperm Motility/genetics Spermatogenesis/genetics Spermatozoa/metabolism Transcriptome/genetics
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Montjean Debbie
Advanced Technology Laboratory, ZA de l'Agiot 4 rue Louis Lormand, 78320, La Verrière, Paris, France. [email protected]
De La Grange Pierre
Gentien David
Rapinat Audrey
Belloc Stéphanie
Cohen-Bacrie Paul
Menezo Yves
Benkhalifa Moncef
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Article Info
Journal
Journal of assisted reproduction and genetics
Abbr.
J Assist Reprod Genet
ISSN
1573-7330
DOI
10.1007/s10815-011-9644-3
Email
[email protected]
Published
2012-01-00
Epub
2011-00-12
Pages
3-10
Language
English
Region
Netherlands
NLM ID
9206495
PMCID
PMC3252406
Subset
IM
External Links
PubMed source DOI: 10.1007/s10815-011-9644-3 PMC Full Text View journal details
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