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PMID: 2199796 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Use of T7 RNA polymerase to direct expression of cloned genes.

Methods in enzymology ·Vol. 185 ·1990-00-00 ·Pages 60-89

Studier FW, Rosenberg AH, Dunn JJ, Dubendorff JW

Abstract

暂无摘要

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular/methods DNA-Directed RNA Polymerases/metabolism Escherichia coli/genetics Gene Expression Genetic Vectors Molecular Sequence Data Nucleic Acid Conformation Plasmids Promoter Regions, Genetic Protein Biosynthesis Restriction Mapping T-Phages/enzymology
Chemicals
DNA-Directed RNA Polymerases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Studier F W
Rosenberg A H
Dunn J J
Dubendorff J W
Article Info
Journal
Methods in enzymology
Abbr.
Methods Enzymol
ISSN
0076-6879
DOI
10.1016/0076-6879(90)85008-c
Published
1990-00-00
Pages
60-89
Language
English
Region
United States
NLM ID
0212271
Subset
IM
Grants
NIGMS NIH HHS · GM21872 · United States
External Links
PubMed source DOI: 10.1016/0076-6879(90)85008-c View journal details
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