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PMID: 22006337 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Determinants of endocytic membrane geometry, stability, and scission.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 108 ·No. 44 ·2011-11-01 ·Pages E979-88

Kishimoto T, Sun Y, Buser C, Liu J, Michelot A, Drubin DG

Abstract

During endocytic vesicle formation, distinct subdomains along the membrane invagination are specified by different proteins, which bend the membrane and drive scission. Bin-Amphiphysin-Rvs (BAR) and Fer-CIP4 homology-BAR (F-BAR) proteins can induce membrane curvature and have been suggested to facilitate membrane invagination and scission. Two F-BAR proteins, Syp1 and Bzz1, are found at budding yeast endocytic sites. Syp1 arrives early but departs from the endocytic site before formation of deep membrane invaginations and scission. Using genetic, spatiotemporal, and ultrastructural analyses, we demonstrate that Bzz1, the heterodimeric BAR domain protein Rvs161/167, actin polymerization, and the lipid phosphatase Sjl2 cooperate, each through a distinct mechanism, to induce membrane scission in yeast. Additionally, actin assembly and Rvs161/167 cooperate to drive formation of deep invaginations. Finally, we find that Bzz1, acting at the invagination base, stabilizes endocytic sites and functions with Rvs161/167, localized along the tubule, to achieve proper endocytic membrane geometry necessary for efficient scission. Together, our results reveal that dynamic interplay between a lipid phosphatase, actin assembly, and membrane-sculpting proteins leads to proper membrane shaping, tubule stabilization, and scission.

MeSH Terms
Amino Acid Sequence Cell Membrane/metabolism,ultrastructure Endocytosis Membrane Proteins/chemistry,metabolism Molecular Sequence Data Saccharomyces cerevisiae/metabolism Sequence Homology, Amino Acid
Chemicals
Membrane Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Kishimoto Takuma
Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720-3202, USA.
Sun Yidi
Buser Christopher
Liu Jian
Michelot Alphée
Drubin David G
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
1091-6490
DOI
10.1073/pnas.1113413108
Published
2011-11-01
Epub
2011-00-17
Pages
E979-88
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC3207701
Subset
IM
Grants
NIGMS NIH HHS · GM R01 42759 · United States
NIGMS NIH HHS · GM R01 50399 · United States
External Links
PubMed source DOI: 10.1073/pnas.1113413108 PMC Full Text View journal details
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