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PMID: 2202594 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Retracted Publication

Differential repair of UV damage in Saccharomyces cerevisiae is cell cycle dependent.

The EMBO journal ·Vol. 9 ·No. 9 ·1990-09-00 ·Pages 2899-904

Terleth C, Waters R, Brouwer J, van de Putte P

Abstract

In the yeast Saccharomyces cerevisiae the transcriptionally active MAT alpha locus is repaired preferentially to the inactive HML alpha locus after UV irradiation. Here we analysed the repair of both loci after irradiating yeast cells at different stages of the mitotic cell cycle. In all stages repair of the active MAT alpha locus occurs at a rate of 30% removal of dimers per hour after a UV dose of 60 J/m2. The inactive HML alpha is repaired as efficiently as MAT alpha following irradiation in G2 whereas repair of HML alpha is less efficient in the other stages. Thus differential repair is observed in G1 and S but not in G2. Apparently, in G2 a chromatin structure exists in which repair does not discriminate between transcriptionally active and inactive DNA or, alternatively, an additional repair mechanism might exist which is only operational during G2.

MeSH Terms
Blotting, Southern Cell Cycle DNA Damage DNA Repair DNA, Fungal/genetics,radiation effects Kinetics Saccharomyces cerevisiae/cytology,genetics,radiation effects Ultraviolet Rays
Chemicals
DNA, Fungal
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Terleth C
Department of Biochemistry, Gorlaeus Laboratories, Leiden University, The Netherlands.
Waters R
Brouwer J
van de Putte P
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28 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1990-09-00
Pages
2899-904
Language
English
Region
England
NLM ID
8208664
PMCID
PMC552005
Subset
IM
Corrections
RetractionIn
PMID: 1547782
External Links
PubMed source PMC Full Text View journal details
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