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PMID: 2205536 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The 5'-leader sequence of tobacco mosaic virus RNA mediates initiation-factor-4E-independent, but still initiation-factor-4A-dependent translation in yeast extracts.

Gene ·Vol. 91 ·No. 1 ·1990-07-02 ·Pages 127-9

Altmann M, Blum S, Wilson TM, Trachsel H

Abstract

Messenger RNAs encoding chloramphenicol acetyltransferase (CAT) with or without the 5'-leader sequence of tobacco mosaic virus (TMV) RNA were synthesized in vitro and translated in Saccharomyces cerevisiae extracts dependent on eukaryotic initiation factors eIF-4E or eIF-4A. The 5'-leader sequence of TMV RNA renders translation of CAT mRNA eIF-4E-independent but still 4A-dependent.

MeSH Terms

Chloramphenicol O-Acetyltransferase/genetics Eukaryotic Initiation Factor-4A Eukaryotic Initiation Factor-4E Methionine/metabolism Peptide Initiation Factors/metabolism Protein Biosynthesis Protein Sorting Signals/genetics RNA, Messenger/genetics RNA, Viral/genetics Saccharomyces cerevisiae/metabolism Tobacco Mosaic Virus/genetics

Chemicals

Eukaryotic Initiation Factor-4E Peptide Initiation Factors Protein Sorting Signals RNA, Messenger RNA, Viral Methionine Chloramphenicol O-Acetyltransferase Eukaryotic Initiation Factor-4A

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Altmann M

Institut für Biochemie und Molekularbiologie, Universität Bern, Switzerland.

Blum S

Wilson T M

Trachsel H

Article Info

Journal

Gene

Abbr.

Gene

ISSN

0378-1119

DOI

10.1016/0378-1119(90)90173-o

Published

1990-07-02

Pages

127-9

Language

English

Region

Netherlands

NLM ID

7706761

Subset

External Links

PubMed source DOI: 10.1016/0378-1119(90)90173-o View journal details

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The 5'-leader sequence of tobacco mosaic virus RNA mediates initiation-factor-4E-independent, but still initiation-factor-4A-dependent translation in yeast extracts.

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