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PMID: 22065898 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Genes associated with recurrence of hepatocellular carcinoma: integrated analysis by gene expression and methylation profiling.

Journal of Korean medical science ·Vol. 26 ·No. 11 ·2011-11-00 ·Pages 1428-38

Yang JD, Seol SY, Leem SH, Kim YH, Sun Z, Lee JS, Thorgeirsson SS, Chu IS, Roberts LR, Kang KJ

Abstract

Gene expression is suppressed by DNA methylation. The goal of this study was to identify genes whose CpG site methylation and mRNA expression are associated with recurrence after surgical resection for hepatocellular carcinoma (HCC). Sixty-two HCCs were examined by both whole genome DNA methylation and transcriptome analysis. The Cox model was used to select genes associated with recurrence. A validation was performed in an independent cohort of 66 HCC patients. Among fifty-nine common genes, increased CpG site methylation and decreased mRNA expression were associated with recurrence for 12 genes (Group A), whereas decreased CpG site methylation and increased mRNA expression were associated with recurrence for 25 genes (Group B). The remaining 22 genes were defined as Group C. Complement factor H (CFH) and myosin VIIA and Rab interacting protein (MYRIP) in Group A; proline/serine-rich coiled-coil 1 (PSRC1), meiotic recombination 11 homolog A (MRE11A), and myosin IE (MYO1E) in Group B; and autophagy-related protein LC3 A (MAP1LC3A), and NADH dehydrogenase 1 alpha subcomplex assembly factor 1 (NDUFAF1) in Group C were validated. In conclusion, potential tumor suppressor (CFH, MYRIP) and oncogenes (PSRC1, MRE11A, MYO1E) in HCC are reported. The regulation of individual genes by methylation in hepatocarcinogenesis needs to be validated.

Keywords
Carcinoma Hepatocellular DNA methylation Gene Expression Profiling Microarray analysis Survival
MeSH Terms
Adult Aged Carcinoma, Hepatocellular/genetics,pathology,surgery CpG Islands DNA Methylation Female Gene Expression Profiling Gene Expression Regulation, Neoplastic Humans Liver/pathology Liver Neoplasms/genetics,pathology,surgery Male Middle Aged Neoplasm Recurrence, Local/genetics Oligonucleotide Array Sequence Analysis Proportional Hazards Models RNA, Messenger/biosynthesis Transcriptome/genetics
Chemicals
RNA, Messenger
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Yang Ju Dong
Miles and Shirley Fiterman Center for Digestive Diseases, College of Medicine, Mayo Clinic, Rochester, MN, USA.
Seol So-Young
Leem Sun-Hee
Kim Yong Hoon
Sun Zhifu
Lee Ju-Seog
Thorgeirsson Snorri S
Chu In-Sun
Roberts Lewis R
Kang Koo Jeong
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Article Info
Journal
Journal of Korean medical science
Abbr.
J Korean Med Sci
ISSN
1598-6357
DOI
10.3346/jkms.2011.26.11.1428
Published
2011-11-00
Epub
2011-00-27
Pages
1428-38
Language
English
Region
Korea (South)
NLM ID
8703518
PMCID
PMC3207045
Subset
IM
Grants
NCI NIH HHS · R01 CA100882 · United States
NCI NIH HHS · CA128633 · United States
NCI NIH HHS · R56 CA100882 · United States
NCI NIH HHS · R21 CA128633 · United States
NCI NIH HHS · CA100882 · United States
External Links
PubMed source DOI: 10.3346/jkms.2011.26.11.1428 PMC Full Text View journal details
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