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PMID: 22075145 Published · ppublish English Journal Article Research Support, N.I.H., Intramural

A highly dynamic ER-derived phosphatidylinositol-synthesizing organelle supplies phosphoinositides to cellular membranes.

Developmental cell ·Vol. 21 ·No. 5 ·2011-11-15 ·Pages 813-24

Kim YJ, Guzman-Hernandez ML, Balla T

Abstract

Polyphosphoinositides are lipid signaling molecules generated from phosphatidylinositol (PtdIns) with critical roles in vesicular trafficking and signaling. It is poorly understood where PtdIns is located within cells and how it moves around between membranes. Here we identify a hitherto-unrecognized highly mobile membrane compartment as the site of PtdIns synthesis and a likely source of PtdIns of all membranes. We show that the PtdIns-synthesizing enzyme PIS associates with a rapidly moving compartment of ER origin that makes ample contacts with other membranes. In contrast, CDP-diacylglycerol synthases that provide PIS with its substrate reside in the tubular ER. Expression of a PtdIns-specific bacterial PLC generates diacylglycerol also in rapidly moving cytoplasmic objects. We propose a model in which PtdIns is synthesized in a highly mobile lipid distribution platform and is delivered to other membranes during multiple contacts by yet-to-be-defined lipid transfer mechanisms.

MeSH Terms
Animals COS Cells Cell Membrane/chemistry,metabolism Chlorocebus aethiops Diacylglycerol Cholinephosphotransferase/chemistry,metabolism Endoplasmic Reticulum/chemistry,enzymology,metabolism HEK293 Cells Humans Organelles/chemistry,metabolism Phosphatidylinositols/biosynthesis,chemistry,metabolism Signal Transduction
Chemicals
Phosphatidylinositols Diacylglycerol Cholinephosphotransferase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kim Yeun Ju
Section on Molecular Signal Transduction, Program for Developmental Neuroscience, NICHD, National Institutes of Health, Bethesda, MD 20892, USA.
Guzman-Hernandez Maria Luisa
Balla Tamas
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Article Info
Journal
Developmental cell
Abbr.
Dev Cell
ISSN
1878-1551
DOI
10.1016/j.devcel.2011.09.005
Published
2011-11-15
Pages
813-24
Language
English
Region
United States
NLM ID
101120028
PMCID
PMC3235737
Subset
IM
Grants
Intramural NIH HHS · Z01 HD000196-10 · United States
Corrections
CommentIn
PMID: 22075144
External Links
PubMed source DOI: 10.1016/j.devcel.2011.09.005 PMC Full Text View journal details
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