Minicell-forming mutants of Escherichia coli: suppression of both DicB- and MinD-dependent division inhibition by inactivation of the minC gene product.
We have determined the nucleotide sequence of the minB operon of 10 min mutants of Escherichia coli, characterized by impaired inhibition of polar divisions. These mutants were either sensitive or resistant to the division inhibitor DicB. All the mutations were found to lie in minC or minD, confirming the requirement of both gene products in the process of inhibition of polar sites. Mutations conferring resistance to inhibitor DicB were found exclusively in minC. In agreement with de Boer et al. (P. A. J. de Boer, R. E. Crossley, and L. I. Rothfield, Proc. Natl. Acad. Sci. USA 87:1129-1133, 1990), these results provide evidence that, in addition to promoting division inhibition with MinD, protein MinC acts in concert with DicB to inhibit division by a second, MinD-independent process.
16 references, click to expand
-
Minicell-forming mutants of Escherichia coli: production of minicells and anucleate rods.J Bacteriol. 1988 Jul;170(7):3094-101 PMID: 2838458
-
Genetic basis of minicell formation in Escherichia coli K-12.J Bacteriol. 1984 Jun;158(3):1202-3 PMID: 6327636
-
Identification and sequence of gene dicB: translation of the division inhibitor from an in-phase internal start.Nucleic Acids Res. 1988 Jul 25;16(14A):6327-38 PMID: 3041373
-
New method for generating deletions and gene replacements in Escherichia coli.J Bacteriol. 1989 Sep;171(9):4617-22 PMID: 2548993
-
MINIATURE escherichia coli CELLS DEFICIENT IN DNA.Proc Natl Acad Sci U S A. 1967 Feb;57(2):321-6 PMID: 16591472
-
The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library.Cell. 1987 Jul 31;50(3):495-508 PMID: 3038334
-
A pSC101-derived plasmid which shows no sequence homology to other commonly used cloning vectors.Gene. 1984 Nov;31(1-3):165-71 PMID: 6098521
-
Quantal behavior of a diffusible factor which initiates septum formation at potential division sites in Escherichia coli.J Bacteriol. 1974 May;118(2):407-13 PMID: 4597442
-
Mutations of temperature sensitivity in R plasmid pSC101.J Bacteriol. 1977 Aug;131(2):405-12 PMID: 328479
-
Genetic mapping of the minB locus in Escherichia coli K-12.J Bacteriol. 1983 Feb;153(2):1063-5 PMID: 6296039
-
Direct cloning of specific genomic DNA sequences in plasmid libraries following fragment enrichment.Nucleic Acids Res. 1985 Nov 11;13(21):7569-78 PMID: 2999697
-
Isolation and mapping of Escherichia coli mutations conferring resistance to division inhibition protein DicB.J Bacteriol. 1989 Aug;171(8):4315-9 PMID: 2666395
-
A division inhibitor and a topological specificity factor coded for by the minicell locus determine proper placement of the division septum in E. coli.Cell. 1989 Feb 24;56(4):641-9 PMID: 2645057
-
Central role for the Escherichia coli minC gene product in two different cell division-inhibition systems.Proc Natl Acad Sci U S A. 1990 Feb;87(3):1129-33 PMID: 2137246
-
Establishing homologies in protein sequences.Methods Enzymol. 1983;91:524-45 PMID: 6855599
-
Isolation and properties of minB, a complex genetic locus involved in correct placement of the division site in Escherichia coli.J Bacteriol. 1988 May;170(5):2106-12 PMID: 2834323
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