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PMID: 22158466 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

The RPT2 subunit of the 26S proteasome directs complex assembly, histone dynamics, and gametophyte and sporophyte development in Arabidopsis.

The Plant cell ·Vol. 23 ·No. 12 ·2011-12-00 ·Pages 4298-317

Lee KH, Minami A, Marshall RS, Book AJ, Farmer LM, Walker JM, Vierstra RD

Abstract

The regulatory particle (RP) of the 26S proteasome contains a heterohexameric ring of AAA-ATPases (RPT1-6) that unfolds and inserts substrates into the core protease (CP) for degradation. Through genetic analysis of the Arabidopsis thaliana gene pair encoding RPT2, we show that this subunit plays a critical role in 26S proteasome assembly, histone dynamics, and plant development. rpt2a rpt2b double null mutants are blocked in both male and female gamete transmission, demonstrating that the subunit is essential. Whereas rpt2b mutants are phenotypically normal, rpt2a mutants display a range of defects, including impaired leaf, root, trichome, and pollen development, delayed flowering, stem fasciation, hypersensitivity to mitomycin C and amino acid analogs, hyposensitivity to the proteasome inhibitor MG132, and decreased 26S complex stability. The rpt2a phenotype can be rescued by both RPT2a and RPT2b, indicative of functional redundancy, but not by RPT2a mutants altered in ATP binding/hydrolysis or missing the C-terminal hydrophobic sequence that docks the RPT ring onto the CP. Many rpt2a phenotypes are shared with mutants lacking the chromatin assembly factor complex CAF1. Like caf1 mutants, plants missing RPT2a or reduced in other RP subunits contain less histones, thus implicating RPT2 specifically, and the 26S proteasome generally, in plant nucleosome assembly.

MeSH Terms
Adenosine Triphosphate/metabolism Alleles Amino Acid Sequence Arabidopsis/drug effects,genetics,growth & development,metabolism Arabidopsis Proteins/genetics,metabolism Cell Fractionation Gene Expression Regulation, Plant Genes, Plant Genetic Complementation Test Genetic Loci Germ Cells, Plant/cytology,growth & development,metabolism Histones/genetics,metabolism Immunoblotting Mitomycin/pharmacology Molecular Sequence Data Mutagenesis, Site-Directed Phenotype Plant Roots/growth & development,metabolism Pollen/genetics,metabolism Proteasome Endopeptidase Complex/genetics,metabolism Sequence Deletion Signal Transduction Transgenes
Chemicals
Arabidopsis Proteins Histones RPT2a protein, Arabidopsis Mitomycin Adenosine Triphosphate Proteasome Endopeptidase Complex ATP dependent 26S protease
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Lee Kwang-Hee
Department of Genetics, University of Wisconsin, Madison, Wisconsin 53706, USA.
Minami Atsushi
Marshall Richard S
Book Adam J
Farmer Lisa M
Walker Joseph M
Vierstra Richard D
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1532-298X
DOI
10.1105/tpc.111.089482
Published
2011-12-00
Epub
2011-00-09
Pages
4298-317
Language
English
Region
England
NLM ID
9208688
PMCID
PMC3269867
Subset
IM
Grants
NINDS NIH HHS · F31 NS054563 · United States
NINDS NIH HHS · 5F31NS054563 · United States
External Links
PubMed source DOI: 10.1105/tpc.111.089482 PMC Full Text View journal details
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