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PMID: 22194901 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Maternal periconceptional and gestational low protein diet affects mouse offspring growth, cardiovascular and adipose phenotype at 1 year of age.

PloS one ·Vol. 6 ·No. 12 ·2011-00-00 ·Pages e28745

Watkins AJ, Lucas ES, Wilkins A, Cagampang FR, Fleming TP

Abstract

Human and animal studies have revealed a strong association between periconceptional environmental factors, such as poor maternal diet, and an increased propensity for cardiovascular and metabolic disease in adult offspring. Previously, we reported cardiovascular and physiological effects of maternal low protein diet (LPD) fed during discrete periods of periconceptional development on 6-month-old mouse offspring. Here, we extend the analysis in 1 year aging offspring, evaluating mechanisms regulating growth and adiposity. Isocaloric LPD (9% casein) or normal protein diet (18% casein; NPD) was fed to female MF-1 mice either exclusively during oocyte maturation (for 3.5 days prior to mating; Egg-LPD, Egg-NPD, respectively), throughout gestation (LPD, NPD) or exclusively during preimplantation development (for 3.5 days post mating; Emb-LPD). LPD and Emb-LPD female offspring were significantly lighter and heavier than NPD females respectively for up to 52 weeks. Egg-LPD, LPD and Emb-LPD offspring displayed significantly elevated systolic blood pressure at 52 weeks compared to respective controls (Egg-NPD, NPD). LPD females had significantly reduced inguinal and retroperitoneal fat pad: body weight ratios compared to NPD females. Expression of the insulin receptor (Insr) and insulin-like growth factor I receptor (Igf1r) in retroperitoneal fat was significantly elevated in Emb-LPD females (P<0.05), whilst Emb-LPD males displayed significantly decreased expression of the mitochondrial uncoupling protein 1 (Ucp1) gene compared to NPD offspring. LPD females displayed significantly increased expression of Ucp1 in interscapular brown adipose tissue when compared to NPD offspring. Our results demonstrate that aging offspring body weight, cardiovascular and adiposity homeostasis can be programmed by maternal periconceptional nutrition. These adverse outcomes further exemplify the criticality of dietary behaviour around the time of conception on long-term offspring health.

MeSH Terms
Adipose Tissue/growth & development,metabolism,pathology Animals Animals, Newborn Blood Glucose/metabolism Blood Pressure Body Weight Cardiovascular System/growth & development,metabolism,pathology Diet, Protein-Restricted Fasting/blood Female Gene Expression Regulation, Developmental Growth and Development/physiology Humans Insulin/blood Male Maternal Nutritional Physiological Phenomena Mice Organ Size Phenotype Pregnancy Prenatal Exposure Delayed Effects/blood,pathology,physiopathology Real-Time Polymerase Chain Reaction
Chemicals
Blood Glucose Insulin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Watkins Adam J
School of Biological Sciences, University of Southampton, Southampton General Hospital, Southampton, United Kingdom. [email protected]
Lucas Emma S
Wilkins Adrian
Cagampang Felino R A
Fleming Tom P
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Article Info
Journal
PloS one
Abbr.
PLoS One
ISSN
1932-6203
DOI
10.1371/journal.pone.0028745
Email
[email protected]
Published
2011-00-00
Epub
2011-00-15
Pages
e28745
Language
English
Region
United States
NLM ID
101285081
PMCID
PMC3240629
Subset
IM
Grants
Biotechnology and Biological Sciences Research Council · BB/F007450/1 · United Kingdom
Biotechnology and Biological Sciences Research Council · BBF007450 · United Kingdom
NICHD NIH HHS · U01 HD04435 · United States
External Links
PubMed source DOI: 10.1371/journal.pone.0028745 PMC Full Text View journal details
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