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PMID: 2222415 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

There are two major types of skeletal keratan sulphates.

The Biochemical journal ·Vol. 271 ·No. 1 ·1990-10-01 ·Pages 243-5

Nieduszynski IA, Huckerby TN, Dickenson JM, Brown GM, Tai GH, Morris HG, Eady S

Abstract

High-field 1H-n.m.r.-spectroscopic studies supported by chemical carbohydrate analyses show that skeletal keratan sulphates (KS-II) of bovine origin may be sub-classified into two groups. Keratan sulphate chains from articular and intervertebral-disc cartilage (KS-II-A) contain two structural features, namely alpha(1----3)-fucose and alpha(2----6)-linked N-acetyl-neuraminic acid residues, that are absent from keratan sulphates from tracheal or nasal-septum cartilage (KS-II-B).

MeSH Terms

Animals Carbohydrate Conformation Cartilage/chemistry Cartilage, Articular/chemistry Cattle Fucose/analysis Galactose/analysis Intervertebral Disc/chemistry Keratan Sulfate/analysis Magnetic Resonance Spectroscopy N-Acetylneuraminic Acid Nasal Septum/chemistry Sialic Acids/analysis Trachea/chemistry

Chemicals

Sialic Acids Fucose Keratan Sulfate N-Acetylneuraminic Acid Galactose

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Nieduszynski I A

Division of Biological Sciences, University of Lancaster, Bailrigg, U.K.

Huckerby T N

Dickenson J M

Brown G M

Tai G H

Morris H G

Eady S

References (10)

10 references, click to expand

Structural studies of two populations of keratan sulphate chains from mature bovine articular cartilage.
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Structure of human skeletal keratosulfate. The linkage region.
J Biol Chem. 1967 Jul 25;242(14):3373-80 PMID: 4226476
Distribution of keratan sulfate in cartilage proteoglycans.
J Biol Chem. 1977 Mar 25;252(6):1971-9 PMID: 139406
Rapid, automated analysis of monosaccharides by high-performance anion-exchange chromatography of borate complexes with fluorimetric detection using 2-cyanoacetamide.
Anal Biochem. 1981 May 1;113(1):130-8 PMID: 7270878
Two linkage-region fragments isolated from skeletal keratan sulphate contain a sulphated N-acetylglucosamine residue.
Biochem J. 1990 Jul 1;269(1):55-9 PMID: 2142881
Identification of an O-glycosidic mannose-linked sialylated tetrasaccharide and keratan sulfate oligosaccharides in the chondroitin sulfate proteoglycan of brain.
J Biol Chem. 1986 Jun 25;261(18):8237-42 PMID: 2941416
Synthesis of small proteoglycans substituted with keratan sulfate by rabbit articular chondrocytes.
J Biol Chem. 1989 Aug 25;264(24):14447-54 PMID: 2527236
Structural and immunological studies of keratan sulphates from mature bovine articular cartilage.
Biochem J. 1989 May 15;260(1):277-82 PMID: 2528344
The keratan sulfate-enriched region of bovine cartilage proteoglycan consists of a consecutively repeated hexapeptide motif.
J Biol Chem. 1989 Sep 25;264(27):16170-3 PMID: 2528543
Analysis by high-performance liquid chromatography of radioactively labeled carbohydrate components of proteoglycans.
Anal Biochem. 1986 Apr;154(1):75-84 PMID: 3706739

Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj2710243

Published

1990-10-01

Pages

243-5

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1149539

Subset

External Links

PubMed source DOI: 10.1042/bj2710243 PMC Full Text View journal details

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There are two major types of skeletal keratan sulphates.

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