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PMID: 22238681 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Expression patterns of non-coding spliced transcripts from human endogenous retrovirus HERV-H elements in colon cancer.

PloS one ·Vol. 7 ·No. 1 ·2012-00-00 ·Pages e29950

Liang Q, Xu Z, Xu R, Wu L, Zheng S

Abstract

Up-regulation of the most abundant H family human endogenous retrovirus (HERV-H), especially env-related transcripts, correlates with colon cancer. However, expression pattern of spliced non-coding transcripts of HERV-H is not clear. In this study, expression of HERV-H spliced transcripts in colon cancer was investigated by a RT-PCR strategy using primers targeting the tRNA(His) primer-binding site and the R region in the 3' long terminal repeat (LTR), followed by cloning and sequencing of the amplicons. Sequences were then assigned to individual HERV-H loci by employing private nucleotide differences between loci. Different expression patterns of HERV-H spliced transcripts from distinct active elements were found in colon cancer cell lines HT29, LS 174T, RKO, SW480 and SW620. Furthermore, the expression patterns in SW480 and RKO were significantly changed by demethylation treatment. Interestingly, more HERV-H elements were found to be transcriptionally active in colon tumor tissues than in adjacent normal tissues (14 vs. 7). This is the first research to study the character of expression of non-coding spliced transcripts of HERV-H elements in colon cancer. Expression patterns of HERV-H spliced transcripts differed among colon cancer cell lines and could be affected by genomic DNA methylation levels. More importantly, besides the commonly accepted view of up-regulation of HERV-H expression in colon tumor tissues, we found more active HERV-H loci in colon tumor as compared with adjacent normal tissues.

MeSH Terms
Adenoma/genetics Alternative Splicing/physiology Base Sequence Carcinoma/genetics Cell Line, Tumor Colonic Neoplasms/genetics Endogenous Retroviruses/genetics Gene Expression Regulation, Neoplastic HT29 Cells Humans Molecular Sequence Data RNA Isoforms/genetics RNA, Untranslated/genetics
Chemicals
RNA Isoforms RNA, Untranslated
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Liang Qiaoyi
Cancer Institute, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.
Xu Zefeng
Xu Rongzhen
Wu Lijun
Zheng Shu
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Article Info
Journal
PloS one
Abbr.
PLoS One
ISSN
1932-6203
DOI
10.1371/journal.pone.0029950
Published
2012-00-00
Epub
2012-00-06
Pages
e29950
Language
English
Region
United States
NLM ID
101285081
PMCID
PMC3253121
Subset
IM
Databases
GENBANK
JK017330, JK017331, JK017332, JK017333, JK017334, JK017335, JK017336, JK017337, JK017338, JK017339, JK017340, JK017341, JK017342, JK017343, JK017344, JK017345, JK017346, JK017347, JK017348, JK017349, JK017350, JK017351, JK017352, JK017353, JK017354, JK017355, JK017356, JK017357, JK017358, JK017359, JK017360, JK017361, JK017362, JK017363, JK017364, JK017365, JK017366, JK017367, JK017368, JK017369, JK017370, JK017371, JK017372, JK017373, JK017374, JK017375, JK017376, JK017377, JK017378, JK017379, JK017380, JK017381, JK017382, JK017383, JK017384, JK017385, JK017386, JK017387, JK017388, JK017389, JK017390, JK017391, JK017392, JK017393, JK017394, JK017395, JK017396, JK017397, JK017398, JK017399, JK017400, JK017401, JK017402, JK017403, JK017404, JK017405, JK017406, JK017407, JK017408, JK017409, JK017410, JK017411, JK017412, JK017413, JK017414, JK017415, JK475044, JK475045, JK475046, JK475047, JK475048, JK475049
External Links
PubMed source DOI: 10.1371/journal.pone.0029950 PMC Full Text View journal details
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