Evidence of a role for TNF-alpha in cytolysis by CD4+, class II MHC-restricted cytotoxic T cells.

Immunology ·Vol. 71 ·No. 2 ·1990-10-00 ·Pages 208-12

Tite JP

Abstract

Variants of the tumour necrosis factor (TNF)-sensitive cell lines BALB/c 3T3 and WEHI-164/13 were generated by selection in high concentrations of recombinant TNF-alpha. The selected cells were highly resistant to cytolysis by both TNF-alpha and TNF-beta (lymphotoxin); however, there was no significant difference in sensitivity to killing by class I major histocompatibility complex (MHC)-restricted cytotoxic T lymphocytes (CTL). Resistance to TNF-mediated lysis was, however, accompanied by increased resistance to cytolysis by CD4+ class II-restricted CTL. Furthermore, cytolysis of WEHI-164/13 cells by CD4+ was completely inhibited by anti-TNF antiserum. These data strongly suggest a role for TNF in killing by CD4+ class II MHC-restricted CTL but not in class I MHC-restricted killing.

MeSH Terms

Animals CD4 Antigens/analysis Cell Line Cytotoxicity, Immunologic Histocompatibility Antigens Class I/immunology Histocompatibility Antigens Class II/immunology Mice Mice, Inbred BALB C T-Lymphocytes, Cytotoxic/immunology Tumor Necrosis Factor-alpha/immunology

Chemicals

CD4 Antigens Histocompatibility Antigens Class I Histocompatibility Antigens Class II Tumor Necrosis Factor-alpha

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Tite J P

Department of Molecular Biology, Wellcome Biotech, Langley Court, Beckenham, Kent, U.K.

References (11)

11 references, click to expand

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Article Info

Journal

Immunology

Abbr.

Immunology

ISSN

0019-2805

Published

1990-10-00

Pages

208-12

Language

English

Region

England

NLM ID

0374672

PMCID

PMC1384305

Subset

External Links

PubMed source PMC Full Text View journal details

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Evidence of a role for TNF-alpha in cytolysis by CD4+, class II MHC-restricted cytotoxic T cells.

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