High-performance liquid chromatography patterns of mycolic acids as criteria for identification of Mycobacterium chelonae, Mycobacterium fortuitum, and Mycobacterium smegmatis.

Journal of clinical microbiology ·Vol. 28 ·No. 9 ·1990-09-00 ·Pages 2094-8

Butler WR, Kilburn JO

Abstract

Rapidly growing mycobacteria of clinical significance were identified by mycolic acids detected with high-performance liquid chromatography. Mycolic acids from whole cells were extracted, derivatized, and detected by a modified high-performance liquid chromatography procedure in less than 3 h. Use of an internal standard allowed differentiation of Mycobacterium chelonae and Mycobacterium fortuitum by comparison of relative retention times. Peak height ratios were used for subidentification of M. chelonae strains; however, M. fortuitum and Mycobacterium smegmatis could not be separated by this system.

MeSH Terms

Chromatography, High Pressure Liquid Culture Media Humans Mycobacterium/analysis,classification,isolation & purification Mycolic Acids/isolation & purification,standards Nontuberculous Mycobacteria/analysis,classification,isolation & purification Reference Standards Species Specificity Temperature

Chemicals

Culture Media Mycolic Acids

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Butler W R

Division of Bacterial Diseases, Centers for Disease Control, Atlanta, Georgia 30333.

Kilburn J O

References (27)

27 references, click to expand

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Article Info

Journal

Journal of clinical microbiology

Abbr.

J Clin Microbiol

ISSN

0095-1137

DOI

10.1128/jcm.28.9.2094-2098.1990

Published

1990-09-00

Pages

2094-8

Language

English

Region

United States

NLM ID

7505564

PMCID

PMC268109

Subset

External Links

PubMed source DOI: 10.1128/jcm.28.9.2094-2098.1990 PMC Full Text View journal details

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High-performance liquid chromatography patterns of mycolic acids as criteria for identification of Mycobacterium chelonae, Mycobacterium fortuitum, and Mycobacterium smegmatis.

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