Home LiteratureArticle Details

PMID: 223367 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Growth of the WW strain of Theiler virus in mouse central nervous system organotypic culture.

Acta neuropathologica ·Vol. 47 ·No. 1 ·1979-06-15 ·Pages 13-9

Wroblewska Z, Kim SU, Sheffield WD, Gilden DH

Abstract

Myelinated organotypic cultures of mouse central nervous system (CNS) provide a favorable milieu for growth of the WW strain of Theiler virus (TV). WW-TV induced a cytopathic effect characterized by neuronal destruction and swelling of myelin sheaths. Tissue culture medium and homogenates of infected cultures produced neurologic disease in mice, and TV was demonstrated in cultures by indirect immunofluorescence and electron microscopy. Ultrastructurally, severe alterations in neurons and the presence of cytoplasmic inclusions containing paracrystalline arrays of 30-nm virus particles were evident in astrocytes. Demyelination appeared to be secondary to lysis of oligodendrocytes by virus. CNS organotypic cultures provide an environment for the study of TV-induced demyelination.

MeSH Terms

Animals Central Nervous System Culture Techniques Cytopathogenic Effect, Viral Enterovirus/growth & development Inclusion Bodies/ultrastructure Mice Microscopy, Electron Myelin Sheath/ultrastructure Neurons/ultrastructure

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Wroblewska Z

Kim S U

Sheffield W D

Gilden D H

References (13)

13 references, click to expand

Demyelinative myelopathy in mice induced by the DA virus.
J Neurol Sci. 1976 Oct;29(2-4):149-60 PMID: 185333
SPONTANEOUS ENCEPHALOMYELITIS OF MICE, A NEW VIRUS DISEASE.
J Exp Med. 1937 Apr 30;65(5):705-19 PMID: 19870629
Reconstituted rattail collagen used as substrate for tissue cultures on coverslips in Maximow slides and roller tubes.
Lab Invest. 1958 Mar-Apr;7(2):134-7 PMID: 13540204
Virus-specific intracytoplasmic inclusions in mouse brain produced by a newly isolated strain of Theiler virus. I. Virologic and morphologic studies.
Lab Invest. 1977 Dec;37(6):595-602 PMID: 202801
Morphological and biochemical development of chick cerebrum cultured in vitro.
Exp Neurol. 1974 Jun;43(3):515-26 PMID: 4133245
Theiler's virus-induced demyelination: prevention by immunosuppression.
Science. 1976 Apr 2;192(4234):62-4 PMID: 176726
Ultrastructure of mumps virus replication in organotypic cultures of hamster choroid plexus.
J Gen Virol. 1976 Feb;30(2):197-205 PMID: 985852
Theiler's virus infection in mice: an unusual biphasic disease process leading to demyelination.
Infect Immun. 1975 May;11(5):1147-55 PMID: 164412
Experimental panencephalitis induced in suckling mice, by parainfluenza type 1 (6/94) virus, II. Virologic studies.
J Neuropathol Exp Neurol. 1976 May;35(3):259-70 PMID: 178834
Herpes virus hominis type 2 and intranuclear tubular structures in organized nervous tissue cultures.
Acta Neuropathol. 1972;22(2):170-9 PMID: 4342986
The effects of measles virus and various strains of SSPE virus on organotypic cultures of nervous tissue.
Acta Neuropathol. 1976 Mar 15;34(2):175-81 PMID: 1258611
Subacute sclerosing panencephalitis virus. Observations on a neuroadapted and non-neuroadapted strain in organotypic central nervous system cultures.
Lab Invest. 1974 Jul;31(1):42-53 PMID: 4843210
Replication of arboviruses in mouse organ cultures. I. Results with Middelburg virus in organ cultures and peritoneal macrophages.
Arch Gesamte Virusforsch. 1974;45(1-2):78-85 PMID: 4370336

Article Info

Journal

Acta neuropathologica

Abbr.

Acta Neuropathol

ISSN

0001-6322

DOI

10.1007/BF00698267

Published

1979-06-15

Pages

13-9

Language

English

Region

Germany

NLM ID

0412041

Subset

External Links

PubMed source DOI: 10.1007/BF00698267 View journal details

Analysis Services

Growth of the WW strain of Theiler virus in mouse central nervous system organotypic culture.

Contact

WeChat Official Account