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PMID: 22379107 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

A mechanosensory system governs myosin II accumulation in dividing cells.

Molecular biology of the cell ·Vol. 23 ·No. 8 ·2012-04-00 ·Pages 1510-23

Kee YS, Ren Y, Dorfman D, Iijima M, Firtel R, Iglesias PA, Robinson DN

Abstract

The mitotic spindle is generally considered the initiator of furrow ingression. However, recent studies suggest that furrows can form without spindles, particularly during asymmetric cell division. In Dictyostelium, the mechanoenzyme myosin II and the actin cross-linker cortexillin I form a mechanosensor that responds to mechanical stress, which could account for spindle-independent contractile protein recruitment. Here we show that the regulatory and contractility network composed of myosin II, cortexillin I, IQGAP2, kinesin-6 (kif12), and inner centromeric protein (INCENP) is a mechanical stress-responsive system. Myosin II and cortexillin I form the core mechanosensor, and mechanotransduction is mediated by IQGAP2 to kif12 and INCENP. In addition, IQGAP2 is antagonized by IQGAP1 to modulate the mechanoresponsiveness of the system, suggesting a possible mechanism for discriminating between mechanical and biochemical inputs. Furthermore, IQGAP2 is important for maintaining spindle morphology and kif12 and myosin II cleavage furrow recruitment. Cortexillin II is not directly involved in myosin II mechanosensitive accumulation, but without cortexillin I, cortexillin II's role in membrane-cortex attachment is revealed. Finally, the mitotic spindle is dispensable for the system. Overall, this mechanosensory system is structured like a control system characterized by mechanochemical feedback loops that regulate myosin II localization at sites of mechanical stress and the cleavage furrow.

MeSH Terms
Cell Division Chromosomal Proteins, Non-Histone/metabolism Dictyostelium/cytology,metabolism Kinesins/metabolism Mechanotransduction, Cellular Microfilament Proteins/metabolism Myosin Type II/metabolism Protozoan Proteins/genetics,metabolism Spindle Apparatus/metabolism,physiology Stress, Physiological ras GTPase-Activating Proteins/metabolism
Chemicals
Chromosomal Proteins, Non-Histone Microfilament Proteins Protozoan Proteins ctxA protein, Dictyostelium discoideum ras GTPase-Activating Proteins Myosin Type II Kinesins
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Kee Yee-Seir
Department of Cell Biology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Ren Yixin
Dorfman Danielle
Iijima Miho
Firtel Richard
Iglesias Pablo A
Robinson Douglas N
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1939-4586
DOI
10.1091/mbc.E11-07-0601
Published
2012-04-00
Epub
2012-00-29
Pages
1510-23
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC3327329
Subset
IM
Grants
NIGMS NIH HHS · R01 GM066817 · United States
NIGMS NIH HHS · R01 GM086704 · United States
NIGMS NIH HHS · R01 GM084015 · United States
NIGMS NIH HHS · GM86704 · United States
NIGMS NIH HHS · GM066817 · United States
External Links
PubMed source DOI: 10.1091/mbc.E11-07-0601 PMC Full Text View journal details
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