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PMID: 224210 Published · ppublish English Journal Article

Transformation-defective mutants of Rous sarcoma virus with longer sizes of genome RNA and their highly frequent occurrences.

Journal of virology ·Vol. 30 ·No. 2 ·1979-05-00 ·Pages 453-61

Yoshida M, Yamashita M, Nomoto A

Abstract

Transformation-defective (td) mutants with different sizes of genomic RNA were isolated from the Prague strain of Rous sarcoma virus, subgroup C(PR-C). All six td viruses (tdTYPR-C) isolated from a single UV-irradiated stock of PR-C (clone 2 of TYPR-C) had slightly longer RNA than did the ordinary class b RNA of tdB77 and Rous-associated virus-7. td viruses spontaneously segregated in uncloned TYPR-C also contained genomic RNA of a size similar to tdTYPR-C RNA. On the other hand, two td mutants isolated from another stock of PR-C (LAPR-C) had the class b RNA. Fingerprint analysis confirmed that tdTYPR-C and tdLAPR-C were derived by deletion from clone 2 of TYPR-C and LAPR-C, respectively, and also showed that clone 2 of TYPR-C had sequences in its genome RNA different from those of LAPR-C, although it gave a fingerprinting pattern similar to the latter. These results strongly suggest that differences between the nucleotide sequences in TYPR-C and LAPR-C RNA may result in different extents of deletion.

MeSH Terms
Avian Sarcoma Viruses/genetics Base Sequence Cell Transformation, Viral Genes, Viral Mutation Oligoribonucleotides/analysis RNA, Viral/analysis,genetics
Chemicals
Oligoribonucleotides RNA, Viral
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Yoshida M
Yamashita M
Nomoto A
References (16)
16 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.30.2.453-461.1979
Published
1979-05-00
Pages
453-61
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC353348
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.30.2.453-461.1979 PMC Full Text View journal details
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