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PMID: 22448259 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Semaphorin 3A suppresses tumor growth and metastasis in mice melanoma model.

PloS one ·Vol. 7 ·No. 3 ·2012-00-00 ·Pages e33633

Chakraborty G, Kumar S, Mishra R, Patil TV, Kundu GC

Abstract

Recent understanding on cancer therapy indicated that targeting metastatic signature or angiogenic switch could be a promising and rational approach to combat cancer. Advancement in cancer research has demonstrated the potential role of various tumor suppressor proteins in inhibition of cancer progression. Current studies have shown that axonal sprouting inhibitor, semaphorin 3A (Sema 3A) acts as a potent suppressor of tumor angiogenesis in various cancer models. However, the function of Sema 3A in regulation of melanoma progression is not well studied, and yet to be the subject of intense investigation. In this study, using multiple in vitro and in vivo approaches we have demonstrated that Sema 3A acts as a potent tumor suppressor in vitro and in vivo mice (C57BL/6) models. Mouse melanoma (B16F10) cells overexpressed with Sema 3A resulted in significant inhibition of cell motility, invasiveness and proliferation as well as suppression of in vivo tumor growth, angiogenesis and metastasis in mice models. Moreover, we have observed that Sema 3A overexpressed melanoma clone showed increased sensitivity towards curcumin and Dacarbazine, anti-cancer agents. Our results demonstrate, at least in part, the functional approach underlying Sema 3A mediated inhibition of tumorigenesis and angiogenesis and a clear understanding of such a process may facilitate the development of novel therapeutic strategy for the treatment of cancer.

MeSH Terms
Animals Antineoplastic Agents/pharmacology Antineoplastic Agents, Alkylating/pharmacology Apoptosis/drug effects Blotting, Western Case-Control Studies Cell Adhesion/drug effects Cell Movement/drug effects Cell Proliferation/drug effects Cells, Cultured Curcumin/pharmacology Dacarbazine/pharmacology Human Umbilical Vein Endothelial Cells/drug effects Humans Immunoenzyme Techniques Liver Neoplasms/metabolism,prevention & control,secondary Lung Neoplasms/metabolism,prevention & control,secondary Male Melanoma, Experimental/metabolism,pathology,prevention & control Mice Mice, Inbred C57BL Neovascularization, Pathologic/prevention & control Phosphorylation/drug effects RNA, Messenger/genetics Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction Semaphorin-3A/genetics,metabolism Wound Healing/drug effects
Chemicals
Antineoplastic Agents Antineoplastic Agents, Alkylating RNA, Messenger Semaphorin-3A Dacarbazine Curcumin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Chakraborty Goutam
National Center for Cell Science (NCCS), NCCS Complex, Pune, India.
Kumar Santosh
Mishra Rosalin
Patil Tushar V
Kundu Gopal C
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Article Info
Journal
PloS one
Abbr.
PLoS One
ISSN
1932-6203
DOI
10.1371/journal.pone.0033633
Published
2012-00-00
Epub
2012-00-20
Pages
e33633
Language
English
Region
United States
NLM ID
101285081
PMCID
PMC3308985
Subset
IM
External Links
PubMed source DOI: 10.1371/journal.pone.0033633 PMC Full Text View journal details
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