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PMID: 224861 Published · ppublish English Journal Article

Calmodulin and myosin light-chain kinase of rabbit fast skeletal muscle.

The Biochemical journal ·Vol. 179 ·No. 1 ·1979-04-01 ·Pages 89-97

Nairn AC, Perry SV

Abstract

1. It is confirmed that myosin light-chain kinase is a protein of mol.wt. about 80,000 that is inactive in the absence of calmodulin. 2. In the presence of 1 mol of calmodulin/mol of kinase 80-90% of the maximal activity is obtained. 3. Crude preparations of the whole light-chain fraction of rabbit fast-skeletal-muscle myosin contain enough calmodulin to activate the enzyme. A method for the preparation of calmodulin-free P light chain is described. 4. A procedure is described for the isolation of calmodulin from rabbit fast skeletal muscle. 5. Rabbit fast-skeletal-muscle calmodulin is indistinguishable from bovine brain calmodulin in its ability to activate myosin light-chain kinase. The other properties of these two proteins are also very similar. 6. Rabbit fast-skeletal-muscle troponin C was about 10% as effective as calmodulin as activator for myosin light-chain kinase. 7. By chromatography on a Sepharose-calmodulin affinity column evidence was obtained for the formation of a Ca2+-dependent complex between calmodulin and myosin light-chain kinase. 8. Troponin I from rabbit fast skeletal muscle and histone IIAS were phosphorylated by fully activated myosin light-chain kinase at about 1% of the rate of the P light chain.

MeSH Terms
Animals Calcium/metabolism Calmodulin/isolation & purification,metabolism,pharmacology Carrier Proteins/metabolism Enzyme Activation/drug effects Muscles/enzymology Myosins Phosphotransferases/metabolism Rabbits Substrate Specificity
Chemicals
Calmodulin Carrier Proteins Phosphotransferases Myosins Calcium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Nairn A C
Perry S V
References (25)
25 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1790089
Published
1979-04-01
Pages
89-97
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1186598
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1790089 PMC Full Text View journal details
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