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PMID: 2249251 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The recombinational hot spot mutation ade6-M26 of Schizosaccharomyces pombe stimulates recombination at sites in a nearby interval.

Current genetics ·Vol. 18 ·No. 3 ·1990-10-00 ·Pages 193-7

Grimm C, Munz P, Kohli J

Abstract

With the help of in vitro constructed intragenic double mutants, we investigated the influence of the recombinational hot spot mutation ade6-M26 on meiotic recombination between two additional ade6 mutations proximal to it. Recombination was stimulated four-fold when M26 was present in a heterozygous condition and ten-fold when homozygous. M26 itself remained unaffected in a substantial number of these events. This indicates that the stimulation can not only be due to a preferred conversion of M26 to wild-type with co-conversion of the second mutation in cis. A model is proposed in which M26 acts as an "entry site" for recombinational enzymes.

Related Genes
ade6
MeSH Terms
Chromosome Mapping Genes, Fungal Heterozygote Meiosis Mutation Recombination, Genetic Schizosaccharomyces/genetics
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Grimm C
Institute of General Microbiology, University of Bern, Switzerland.
Munz P
Kohli J
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22 references, click to expand
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Article Info
Journal
Current genetics
Abbr.
Curr Genet
ISSN
0172-8083
DOI
10.1007/BF00318379
Published
1990-10-00
Pages
193-7
Language
English
Region
United States
NLM ID
8004904
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00318379 View journal details
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