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PMID: 2251113 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Initiation of transcription of the erythroid promoter of the porphobilinogen deaminase gene is regulated by a cis-acting sequence around the cap site.

Nucleic acids research ·Vol. 18 ·No. 22 ·1990-11-25 ·Pages 6509-15

Beaupain D, Eléouët JF, Roméo PH

Abstract

Although the erythroid-specific promoter of human porphobilinogen deaminase [PBGD] gene has no TATA box, transcription is initiated at a single nucleotide. Using 5' and 3' deletions and point mutations, we have identified an element, located around the initiation site, which is necessary and sufficient for 'in vitro' accurate initiation of transcription. This 15 bp element extends 1 bp 5' and 14 bp 3' from the initiation site. It is composed of two regions, a proximal region centred on the cap site and a distal region which bears homology with the TdT initiator element. We show that a nuclear factor, present both in erythroid and non erythroid cells, binds the distal PBGD initiator element. Lack of heat inactivation suggests that initiation of transcription mediated by this element is not TFIID dependent. By transfection into erythroid cells, we also show that the proximal PBGD initiator element is essential for the selection of the initiation site but not for the regulation of transcription of the PBGD erythroid promoter during erythroid differentiation.

MeSH Terms
Animals Base Sequence DNA/chemistry Erythroid Precursor Cells/enzymology Gene Expression Regulation HeLa Cells Humans Hydroxymethylbilane Synthase/biosynthesis,genetics Mice Molecular Sequence Data Mutation Promoter Regions, Genetic RNA Caps/genetics Regulatory Sequences, Nucleic Acid Sequence Homology, Nucleic Acid Transcription, Genetic
Chemicals
RNA Caps DNA Hydroxymethylbilane Synthase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Beaupain D
INSERM U 91, Hôpital Henri Mondor, Créteil, France.
Eléouët J F
Roméo P H
References (29)
29 references, click to expand
  1. Formation of stable preinitiation complexes between eukaryotic class B transcription factors and promoter sequences.
    Nature. 1983 Feb 24;301(5902):680-6 PMID: 6828151
  2. Structure of the 5' flanking region of the gene encoding human glycophorin A and analysis of its multiple transcripts.
    Gene. 1989 Dec 28;85(2):471-7 PMID: 2628178
  3. Three regions upstream from the cap site are required for efficient and accurate transcription of the rabbit beta-globin gene in mouse 3T6 cells.
    Cell. 1983 Mar;32(3):695-706 PMID: 6299573
  4. Compilation and analysis of Escherichia coli promoter DNA sequences.
    Nucleic Acids Res. 1983 Apr 25;11(8):2237-55 PMID: 6344016
  5. Differences in human alpha- and beta-globin gene expression in mouse erythroleukemia cells: the role of intragenic sequences.
    Cell. 1984 Aug;38(1):251-63 PMID: 6205764
  6. Positionally exact initiation is required for the formation of a stable RNA polymerase II transcription complex in vivo.
    EMBO J. 1988 Dec 1;7(12):3785-92 PMID: 3208751
  7. The "initiator" as a transcription control element.
    Cell. 1989 Apr 7;57(1):103-13 PMID: 2467742
  8. Yeast mRNA initiation sites are determined primarily by specific sequences, not by the distance from the TATA element.
    EMBO J. 1985 Dec 1;4(12):3273-80 PMID: 3912167
  9. Fine structure genetic analysis of a beta-globin promoter.
    Science. 1986 May 2;232(4750):613-8 PMID: 3457470
  10. Molecular cloning and complete primary sequence of human erythrocyte porphobilinogen deaminase.
    Nucleic Acids Res. 1986 Aug 11;14(15):5955-68 PMID: 2875434
  11. Tissue-specific in vitro transcription from the mouse albumin promoter.
    Cell. 1986 Dec 5;47(5):767-76 PMID: 3779841
  12. CAT constructions with multiple unique restriction sites for the functional analysis of eukaryotic promoters and regulatory elements.
    Nucleic Acids Res. 1987 Jul 10;15(13):5490 PMID: 3037497
  13. Structure of the gene for human uroporphyrinogen decarboxylase.
    Nucleic Acids Res. 1987 Sep 25;15(18):7343-56 PMID: 3658695
  14. Alternative transcription and splicing of the human porphobilinogen deaminase gene result either in tissue-specific or in housekeeping expression.
    Proc Natl Acad Sci U S A. 1988 Jan;85(1):6-10 PMID: 3422427
  15. Transcription elements and factors of RNA polymerase B promoters of higher eukaryotes.
    CRC Crit Rev Biochem. 1988;23(2):77-120 PMID: 3048889
  16. Factors involved in specific transcription by mammalian RNA polymerase II: purification, genetic specificity, and TATA box-promoter interactions of TFIID.
    Mol Cell Biol. 1988 Oct;8(10):4028-40 PMID: 3185540
  17. Human alpha-globin gene expression following chromosomal dependent gene transfer into mouse erythroleukemia cells.
    Cell. 1978 Sep;15(1):55-63 PMID: 279411
  18. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  19. In vivo sequence requirements of the SV40 early promotor region.
    Nature. 1981 Mar 26;290(5804):304-10 PMID: 6259538
  20. Promoter specificity and modulation of RNA polymerase II transcription.
    FASEB J. 1989 Apr;3(6):1723-33 PMID: 2649403
  21. The human beta-globin promoter; nuclear protein factors and erythroid specific induction of transcription.
    EMBO J. 1988 Dec 20;7(13):4203-12 PMID: 3243278
  22. Regulated expression of the overlapping ubiquitous and erythroid transcription units of the human porphobilinogen deaminase (PBG-D) gene introduced into non-erythroid and erythroid cells.
    J Biol Chem. 1989 Jun 15;264(17):10186-92 PMID: 2542327
  23. Cloning of cDNA for the major DNA-binding protein of the erythroid lineage through expression in mammalian cells.
    Nature. 1989 Jun 8;339(6224):446-51 PMID: 2725678
  24. Cis- and trans-acting elements involved in the regulation of the erythroid promoter of the human porphobilinogen deaminase gene.
    Proc Natl Acad Sci U S A. 1989 Sep;86(17):6548-52 PMID: 2771941
  25. Structure of a mouse erythroid 5-aminolevulinate synthase gene and mapping of erythroid-specific DNAse I hypersensitive sites.
    Nucleic Acids Res. 1989 Sep 12;17(17):7013-28 PMID: 2780318
  26. Initiation of transcription by RNA polymerase II.
    Biochim Biophys Acta. 1989 Sep 21;1009(1):1-10 PMID: 2675975
  27. Transcription initiation from the dihydrofolate reductase promoter is positioned by HIP1 binding at the initiation site.
    Mol Cell Biol. 1990 Feb;10(2):653-61 PMID: 2300058
  28. Functional dissection of a mouse ribosomal protein promoter: significance of the polypyrimidine initiator and an element in the TATA-box region.
    Proc Natl Acad Sci U S A. 1990 Feb;87(4):1526-30 PMID: 2304915
  29. Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.
    Nucleic Acids Res. 1983 Mar 11;11(5):1475-89 PMID: 6828386
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/18.22.6509
Published
1990-11-25
Pages
6509-15
Language
English
Region
England
NLM ID
0411011
PMCID
PMC332603
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/18.22.6509 PMC Full Text View journal details
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