A simple immunofluorescent technique for the detection of human rotavirus.

Journal of medical virology ·Vol. 3 ·No. 3 ·1979-00-00 ·Pages 189-94

Moosai RB, Gardner PS, Almeida JD, Greenaway MA

Abstract

If trypsin is incorporated in the tissue culture medium it is possible to carry out a sensitive immunofluorescence assay for the presence of human rotavirus. The enhanced effect of trypsin is negated by serum. It has also been established that naturally occurring enzymes in faeces enable some virus to penetrate tissue culture cells. The role of these naturally occurring enzymes in the pathogenesis of rotavirus infection is discussed.

MeSH Terms

Animals Cell Line Child Culture Media Diagnosis, Differential Feces/microbiology Fluorescent Antibody Technique Gastroenteritis/diagnosis Haplorhini Humans Kidney Macaca RNA Viruses/isolation & purification Rotavirus/isolation & purification Trypsin/pharmacology Virus Diseases/diagnosis

Chemicals

Culture Media Trypsin

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Moosai R B

Gardner P S

Almeida J D

Greenaway M A

Article Info

Journal

Journal of medical virology

Abbr.

J Med Virol

ISSN

0146-6615

DOI

10.1002/jmv.1890030304

Published

1979-00-00

Pages

189-94

Language

English

Region

United States

NLM ID

7705876

Subset

External Links

PubMed source DOI: 10.1002/jmv.1890030304 View journal details

Analysis Services

A simple immunofluorescent technique for the detection of human rotavirus.

Contact

WeChat Official Account