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PMID: 225527 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Size of infectious DNA from human and murine cytomegaloviruses.

Journal of virology ·Vol. 30 ·No. 1 ·1979-04-00 ·Pages 414-6

Lakeman AD, Osborn JE

Abstract

Viral DNA was isolated from human and murine cytomegalovirus by equilibrium centrifugation in cesium chloride gradients. The size of the DNA was measured relative to T4 DNA by velocity sedimentation in neutral glycerol gradients, and fractions were assayed for infectious DNA. Infectious murine cytomegalovirus DNA sedimented as a single peak with an estimated molecular weight of 136 X 10(6). Infectious human cytomegalovirus DNA was detected in two peaks with molecular weights of 130 X 10(6) and 150 X 10(6).

MeSH Terms
Animals Cytomegalovirus/analysis,growth & development DNA, Viral/analysis Mice Molecular Weight Nucleic Acid Conformation
Chemicals
DNA, Viral
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Lakeman A D
Osborn J E
References (14)
14 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.30.1.414-416.1979
Published
1979-04-00
Pages
414-6
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC353339
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.30.1.414-416.1979 PMC Full Text View journal details
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