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PMID: 22588898 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Chimeras taking shape: potential functions of proteins encoded by chimeric RNA transcripts.

Genome research ·Vol. 22 ·No. 7 ·2012-07-00 ·Pages 1231-42

Frenkel-Morgenstern M, Lacroix V, Ezkurdia I, Levin Y, Gabashvili A, Prilusky J, Del Pozo A, Tress M, Johnson R, Guigo R, Valencia A

Abstract

Chimeric RNAs comprise exons from two or more different genes and have the potential to encode novel proteins that alter cellular phenotypes. To date, numerous putative chimeric transcripts have been identified among the ESTs isolated from several organisms and using high throughput RNA sequencing. The few corresponding protein products that have been characterized mostly result from chromosomal translocations and are associated with cancer. Here, we systematically establish that some of the putative chimeric transcripts are genuinely expressed in human cells. Using high throughput RNA sequencing, mass spectrometry experimental data, and functional annotation, we studied 7424 putative human chimeric RNAs. We confirmed the expression of 175 chimeric RNAs in 16 human tissues, with an abundance varying from 0.06 to 17 RPKM (Reads Per Kilobase per Million mapped reads). We show that these chimeric RNAs are significantly more tissue-specific than non-chimeric transcripts. Moreover, we present evidence that chimeras tend to incorporate highly expressed genes. Despite the low expression level of most chimeric RNAs, we show that 12 novel chimeras are translated into proteins detectable in multiple shotgun mass spectrometry experiments. Furthermore, we confirm the expression of three novel chimeric proteins using targeted mass spectrometry. Finally, based on our functional annotation of exon organization and preserved domains, we discuss the potential features of chimeric proteins with illustrative examples and suggest that chimeras significantly exploit signal peptides and transmembrane domains, which can alter the cellular localization of cognate proteins. Taken together, these findings establish that some chimeric RNAs are translated into potentially functional proteins in humans.

MeSH Terms
Amino Acid Sequence Cell Membrane/genetics,metabolism Databases, Nucleic Acid Exons Gene Expression Regulation Genome, Human High-Throughput Nucleotide Sequencing Humans Mass Spectrometry/methods Molecular Sequence Annotation Molecular Sequence Data Mutant Chimeric Proteins/genetics,metabolism Organ Specificity Protein Biosynthesis Protein Sorting Signals Protein Structure, Secondary Protein Structure, Tertiary Proteomics/methods RNA, Messenger/genetics,metabolism Sequence Analysis, RNA/methods Structure-Activity Relationship
Chemicals
Mutant Chimeric Proteins Protein Sorting Signals RNA, Messenger
Authors & Affiliations
11 authors, click to expand affiliations / ORCID
Frenkel-Morgenstern Milana
Structural Biology and BioComputing Program, Spanish National Cancer Research Centre, Madrid, Spain;
Lacroix Vincent
Ezkurdia Iakes
Levin Yishai
Gabashvili Alexandra
Prilusky Jaime
Del Pozo Angela
Tress Michael
Johnson Rory
Guigo Roderic
Valencia Alfonso
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Article Info
Journal
Genome research
Abbr.
Genome Res
ISSN
1549-5469
Published
2012-07-00
Epub
2012-00-15
Pages
1231-42
Language
English
Region
United States
NLM ID
9518021
PMCID
PMC3396365
Subset
IM
Grants
NHGRI NIH HHS · U54 HG004555 · United States
NHGRI NIH HHS · U54 HG004557 · United States
Corrections
CommentIn
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