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PMID: 2259622 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Human major histocompatibility complex contains a new cluster of genes between the HLA-D and complement C4 loci.

Nucleic acids research ·Vol. 18 ·No. 24 ·1990-12-25 ·Pages 7251-7

Kendall E, Sargent CA, Campbell RD

Abstract

A new cluster of genes has been defined in the human Major Histocompatibility Complex class III region. The seven novel genes, G12 to G18, are localised in a 160 kb segment of DNA extending from the complement gene cluster towards HLA-DR. The genes were identified by isolation of cDNA clones using cosmid genomic inserts as hybridisation probes, and by the detection of the corresponding transcripts in Northern blot analysis. Characterisation of the cosmid genomic DNA inserts, in conjunction with pulsed field gel electrophoresis analysis of uncloned DNA, for the presence of clustered sites for infrequently cutting restriction endonucleases has revealed that at least 5 of the 7 genes are associated with HTF-islands. These unmethylated CpG-rich sequences are frequently found at the 5' ends of ubiquitously expressed genes. Together with previously published data 36 genes have now been defined in a 680kb stretch of DNA within the MHC. With one gene approximately every 20kb of DNA this represents the most densely packed region of the human genome so far characterised, and is of major significance in relation to the mapping and sequence analysis of the rest of the genome.

Related Genes
G12 G13 G14 G15 G16 G17 G18
MeSH Terms
Blotting, Northern Blotting, Southern Chromosome Walking Cloning, Molecular Complement C4/genetics Cosmids Dinucleoside Phosphates/genetics Genetic Linkage HLA-D Antigens/genetics Humans Major Histocompatibility Complex/genetics Multigene Family Restriction Mapping Transcription, Genetic
Chemicals
Complement C4 Dinucleoside Phosphates HLA-D Antigens cytidylyl-3'-5'-guanosine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kendall E
Department of Biochemistry, University of Oxford, UK.
Sargent C A
Campbell R D
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38 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/18.24.7251
Published
1990-12-25
Pages
7251-7
Language
English
Region
England
NLM ID
0411011
PMCID
PMC332860
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/18.24.7251 PMC Full Text View journal details
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