Home LiteratureArticle Details
PMID: 2263603 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

NF-kappa B: a family of inducible and differentially expressed enhancer-binding proteins in human T cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 24 ·1990-12-00 ·Pages 10028-32

Molitor JA, Walker WH, Doerre S, Ballard DW, Greene WC

Abstract

A sensitive DNA-protein crosslinking approach has been used to characterize four inducible T-cell proteins (50 kDa, 55 kDa, 75 kDa, and 85 kDa) that specifically bind to kappa B enhancer elements. Partial proteolytic mapping revealed a distinct cleavage pattern for three of these proteins. These polypeptides are sequestered as inactive precursors in the cytosol of unstimulated T cells but can be converted into active forms in vivo by phorbol ester stimulation or in vitro by detergent treatment. The induction of these proteins by phorbol ester results in a strikingly biphasic pattern of nuclear expression with the 55-kDa and 75-kDa species appearing within minutes, whereas the 50-kDa and 85-kDa species appear only several hours after cellular stimulation. These data suggest that NF-kappa B-binding activity may not correspond to a single polypeptide but rather a family of at least four inducible and differentially regulated DNA-binding proteins that are expressed with distinct kinetics in human T lymphocytes.

MeSH Terms
Base Sequence Cell Line Cell Nucleus/metabolism Cytosol/metabolism Enhancer Elements, Genetic Gene Expression/drug effects HeLa Cells/metabolism Humans Molecular Sequence Data Molecular Weight NF-kappa B/biosynthesis,genetics Oligonucleotide Probes Peptide Mapping Protein Binding T-Lymphocytes/metabolism Tetradecanoylphorbol Acetate/pharmacology
Chemicals
NF-kappa B Oligonucleotide Probes Tetradecanoylphorbol Acetate
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Molitor J A
Howard Hughes Medical Institute, Department of Medicine, Duke University Medical Center, Durham, NC 27710.
Walker W H
Doerre S
Ballard D W
Greene W C
References (39)
39 references, click to expand
  1. Solubilization of active opiate receptors.
    Proc Natl Acad Sci U S A. 1980 Aug;77(8):4623-7 PMID: 6254034
  2. Activation of the interleukin-2 receptor alpha gene: regulatory role for DNA-protein interactions flanking the kappa B enhancer.
    New Biol. 1989 Oct;1(1):83-92 PMID: 2518691
  3. Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.
    Nucleic Acids Res. 1983 Mar 11;11(5):1475-89 PMID: 6828386
  4. A new method for partial peptide mapping using N-chlorosuccinimide/urea and peptide silver staining in sodium dodecyl sulfate-polyacrylamide gels.
    Anal Biochem. 1982 Dec;127(2):453-7 PMID: 6187241
  5. Multiple nuclear factors interact with the immunoglobulin enhancer sequences.
    Cell. 1986 Aug 29;46(5):705-16 PMID: 3091258
  6. Inducibility of kappa immunoglobulin enhancer-binding protein Nf-kappa B by a posttranslational mechanism.
    Cell. 1986 Dec 26;47(6):921-8 PMID: 3096580
  7. The role of the kappa enhancer and its binding factor NF-kappa B in the developmental regulation of kappa gene transcription.
    Cell. 1987 Jan 16;48(1):121-8 PMID: 3098435
  8. An inducible transcription factor activates expression of human immunodeficiency virus in T cells.
    Nature. 1987 Apr 16-22;326(6114):711-3 PMID: 3031512
  9. A common positive trans-acting factor binds to enhancer sequences in the promoters of mouse H-2 and beta 2-microglobulin genes.
    Proc Natl Acad Sci U S A. 1987 May;84(9):2653-7 PMID: 3554244
  10. Characterization of cellular proteins recognizing the HIV enhancer using a microscale DNA-affinity precipitation assay.
    Nature. 1987 Nov 26-Dec 2;330(6146):391-5 PMID: 2825023
  11. Purification and properties of Drosophila heat shock activator protein.
    Science. 1987 Nov 27;238(4831):1247-53 PMID: 3685975
  12. Two transcription factors, NF-kappa B and H2TF1, interact with a single regulatory sequence in the class I major histocompatibility complex promoter.
    Proc Natl Acad Sci U S A. 1988 Feb;85(3):723-7 PMID: 3422454
  13. Activation of DNA-binding activity in an apparently cytoplasmic precursor of the NF-kappa B transcription factor.
    Cell. 1988 Apr 22;53(2):211-7 PMID: 3129195
  14. The same inducible nuclear proteins regulates mitogen activation of both the interleukin-2 receptor-alpha gene and type 1 HIV.
    Cell. 1988 Jun 3;53(5):827-36 PMID: 2836068
  15. HTLV-1 transactivator induces interleukin-2 receptor expression through an NF-kappa B-like factor.
    Nature. 1988 Jun 23;333(6175):776-8 PMID: 2838755
  16. Identification and purification of a human immunoglobulin-enhancer-binding protein (NF-kappa B) that activates transcription from a human immunodeficiency virus type 1 promoter in vitro.
    Proc Natl Acad Sci U S A. 1988 Jul;85(13):4700-4 PMID: 3133660
  17. Cellular transcription factors and regulation of IL-2 receptor gene expression by HTLV-I tax gene product.
    Science. 1988 Jul 1;241(4861):89-92 PMID: 2838905
  18. HTLV-I tax induces cellular proteins that activate the kappa B element in the IL-2 receptor alpha gene.
    Science. 1988 Sep 23;241(4873):1652-5 PMID: 2843985
  19. How eukaryotic transcriptional activators work.
    Nature. 1988 Oct 20;335(6192):683-9 PMID: 3050531
  20. I kappa B: a specific inhibitor of the NF-kappa B transcription factor.
    Science. 1988 Oct 28;242(4878):540-6 PMID: 3140380
  21. NF-kappa B protein purification from bovine spleen: nucleotide stimulation and binding site specificity.
    Proc Natl Acad Sci U S A. 1988 Dec;85(23):8825-9 PMID: 2848241
  22. Tumor necrosis factor alpha and interleukin 1 stimulate the human immunodeficiency virus enhancer by activation of the nuclear factor kappa B.
    Proc Natl Acad Sci U S A. 1989 Apr;86(7):2336-40 PMID: 2494664
  23. Tumor necrosis factor-alpha activation of the IL-2 receptor-alpha gene involves the induction of kappa B-specific DNA binding proteins.
    J Immunol. 1989 May 1;142(9):3121-8 PMID: 2785134
  24. T-cell activation signals and human T-cell leukemia virus type I-encoded p40x protein activate the mouse granulocyte-macrophage colony-stimulating factor gene through a common DNA element.
    Mol Cell Biol. 1988 Dec;8(12):5581-7 PMID: 2854202
  25. Kappa B-specific DNA binding proteins: role in the regulation of human interleukin-2 gene expression.
    Science. 1989 Apr 28;244(4903):457-60 PMID: 2497518
  26. Induction of interleukin-2 receptor-alpha gene expression is regulated by post-translational activation of kappa B specific DNA binding proteins.
    J Biol Chem. 1989 May 25;264(15):8475-8 PMID: 2785992
  27. NF-kappa B: a pleiotropic mediator of inducible and tissue-specific gene control.
    Cell. 1989 Jul 28;58(2):227-9 PMID: 2665943
  28. Eukaryotic transcriptional regulatory proteins.
    Annu Rev Biochem. 1989;58:799-839 PMID: 2673023
  29. HIV-1, HTLV-1 and normal T-cell growth: transcriptional strategies and surprises.
    Immunol Today. 1989 Aug;10(8):272-8 PMID: 2679647
  30. The SV40 TC-II(kappa B) enhanson binds ubiquitous and cell type specifically inducible nuclear proteins from lymphoid and non-lymphoid cell lines.
    EMBO J. 1989 Dec 20;8(13):4215-27 PMID: 2556265
  31. A 65-kappaD subunit of active NF-kappaB is required for inhibition of NF-kappaB by I kappaB.
    Genes Dev. 1989 Nov;3(11):1689-98 PMID: 2691328
  32. NF-kappa B as inducible transcriptional activator of the granulocyte-macrophage colony-stimulating factor gene.
    Mol Cell Biol. 1990 Mar;10(3):1281-6 PMID: 2406568
  33. Activation in vitro of NF-kappa B by phosphorylation of its inhibitor I kappa B.
    Nature. 1990 Apr 12;344(6267):678-82 PMID: 2157987
  34. Purified human I kappa B can rapidly dissociate the complex of the NF-kappa B transcription factor with its cognate DNA.
    Cell. 1990 Apr 20;61(2):255-65 PMID: 2184941
  35. The DNA binding subunit of NF-kappa B is identical to factor KBF1 and homologous to the rel oncogene product.
    Cell. 1990 Sep 7;62(5):1007-18 PMID: 2203531
  36. Cloning of the p50 DNA binding subunit of NF-kappa B: homology to rel and dorsal.
    Cell. 1990 Sep 7;62(5):1019-29 PMID: 2203532
  37. The v-rel oncogene encodes a kappa B enhancer binding protein that inhibits NF-kappa B function.
    Cell. 1990 Nov 16;63(4):803-14 PMID: 2225078
  38. Involvement of a common transcription factor in the regulated expression of IL-2 and IL-2 receptor genes.
    Int Immunol. 1989;1(1):43-9 PMID: 2518655
  39. Direct activation of calcium-activated, phospholipid-dependent protein kinase by tumor-promoting phorbol esters.
    J Biol Chem. 1982 Jul 10;257(13):7847-51 PMID: 7085651
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.87.24.10028
Published
1990-12-00
Pages
10028-32
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC55308
Subset
IM
Grants
NIGMS NIH HHS · T32-GM07184 · United States
NIGMS NIH HHS · T32-GM07337 · United States
External Links
PubMed source DOI: 10.1073/pnas.87.24.10028 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2