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PMID: 2269474 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Re-evaluation of the chemotactic activity of tumour necrosis factor for monocytes.

Immunology ·Vol. 71 ·No. 3 ·1990-11-00 ·Pages 364-7

Wang JM, Walter S, Mantovani A

Abstract

The present study was designed to re-evaluate the chemotactic activity of recombinant tumour necrosis factor (TNF) and lymphotoxin (LT). TNF and LT induced migration of human monocytes across polycarbonate filter in a 90-min assay. Preincubation with FMLP rendered monocytes unresponsive to subsequent exposure to the same chemoattractant. FMLP-desensitized monocytes retained full responsiveness when exposed to TNF as chemotactic stimulus. The converse was observed when monocytes were deactivated specifically to TNF. FMLP receptor antagonists did not interfere with TNF chemotaxis. A monoclonal anti-TNF antibody inhibited the chemotactic activity of recombinant TNF. These results indicate that, under the experimental conditions utilized here, TNF elicits chemotaxis of human monocytes.

MeSH Terms
Cells, Cultured Chemotaxis, Leukocyte/immunology Humans Lymphotoxin-alpha/immunology Monocytes/immunology N-Formylmethionine Leucyl-Phenylalanine/immunology Recombinant Proteins/immunology Tumor Necrosis Factor-alpha/immunology
Chemicals
Lymphotoxin-alpha Recombinant Proteins Tumor Necrosis Factor-alpha N-Formylmethionine Leucyl-Phenylalanine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Wang J M
Istituto di Ricerche Farmacologiche, Mario Negri, Milan, Italy.
Walter S
Mantovani A
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27 references, click to expand
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Article Info
Journal
Immunology
Abbr.
Immunology
ISSN
0019-2805
Published
1990-11-00
Pages
364-7
Language
English
Region
England
NLM ID
0374672
PMCID
PMC1384433
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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