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PMID: 2269657 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Human fur gene encodes a yeast KEX2-like endoprotease that cleaves pro-beta-NGF in vivo.

The Journal of cell biology ·Vol. 111 ·No. 6 Pt 2 ·1990-12-00 ·Pages 2851-9

Bresnahan PA, Leduc R, Thomas L, Thorner J, Gibson HL, Brake AJ, Barr PJ, Thomas G

Abstract

Extracts from BSC-40 cells infected with vaccinia recombinants expressing either the yeast KEX2 prohormone endoprotease or a human structural homologue (fur gene product) contained an elevated level of a membrane-associated endoproteolytic activity that could cleave at pairs of basic amino acids (-LysArg- and -ArgArg-). The fur-directed activity (furin) shared many properties with Kex2p including activity at pH 7.3 and a requirement for calcium. By using antifurin antibodies, immunoblot analysis detected two furin translation products (90 and 96 kD), while immunofluorescence indicated localization to the Golgi apparatus. Coexpression of either Kex2p or furin with the mouse beta-nerve growth factor precursor (pro-beta-NGF) resulted in greatly enhanced conversion of the precursor to mature nerve growth factor. Thus, the sequence homology shared by furin and the yeast KEX2 prohormone processing enzyme is reflected by significant functional homology both in vitro and in vivo.

MeSH Terms
Amino Acid Sequence Endopeptidases/genetics,physiology Fungal Proteins/genetics Furin Golgi Apparatus/enzymology Humans Membrane Proteins/genetics,physiology Molecular Sequence Data Nerve Growth Factors/metabolism Proprotein Convertases Protein Precursors/metabolism Saccharomyces cerevisiae/enzymology Saccharomyces cerevisiae Proteins Serine Endopeptidases/physiology Substrate Specificity Subtilisins/genetics,physiology
Chemicals
Fungal Proteins Membrane Proteins Nerve Growth Factors Protein Precursors Saccharomyces cerevisiae Proteins Endopeptidases Proprotein Convertases Serine Endopeptidases Subtilisins KEX2 protein, S cerevisiae Furin
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Bresnahan P A
Vollum Institute, Oregon Health Sciences University, Portland 97201-3098.
Leduc R
Thomas L
Thorner J
Gibson H L
Brake A J
Barr P J
Thomas G
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.111.6.2851
Published
1990-12-00
Pages
2851-9
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2116363
Subset
IM
Grants
NIDDK NIH HHS · DK-37274 · United States
NIGMS NIH HHS · GM-21841 · United States
NCRR NIH HHS · RR 07238 · United States
External Links
PubMed source DOI: 10.1083/jcb.111.6.2851 PMC Full Text View journal details
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