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PMID: 226975 Published · ppublish English Journal Article

Interrupting the early region of polyoma virus DNA enhances tumorigenicity.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 8 ·1979-08-00 ·Pages 3713-6

Israel MA, Simmons DT, Hourihan SL, Rowe WP, Martin MA

Abstract

The tumorigenicity of DNA from polyoma virus after cleavage with a variety of restriction enzymes was evaluated in suckling hamsters. Cleavage with enzymes that interrupt the region of the genome coding for the large tumor (T) antigen of polyoma virus markedly enhanced the tumorigenicity above that observed with DNA I of the virus. Cell lines established in vitro from tumors induced by polyoma virions, polyoma virus DNA I, or polyoma virus DNA that had been cleaved with restriction endonucleases in the early region all contain the polyoma virus middle and small T antigens but not the large T antigen of polyoma virus is not required for maintenance of the transformed state and probably not for initiation of tumorigenesis by viral DNA.

MeSH Terms
Animals Antigens, Neoplasm/genetics Base Sequence Cell Transformation, Viral Cricetinae DNA, Viral/genetics Genes, Viral Molecular Weight Neoplasms, Experimental/microbiology Polyomavirus/genetics,pathogenicity Viral Proteins/genetics
Chemicals
Antigens, Neoplasm DNA, Viral Viral Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Israel M A
Simmons D T
Hourihan S L
Rowe W P
Martin M A
References (30)
30 references, click to expand
  1. CELL-TRANSFORMING ABILITY OF A TEMPERATURE-SENSITIVE MUTANT OF POLYOMA VIRUS.
    Proc Natl Acad Sci U S A. 1965 Mar;53:486-91 PMID: 14338226
  2. ISOLATION OF TEMPERATURE-SENSITIVE MUTANTS OF POLYOMA VIRUS.
    Virology. 1965 Apr;25:669-71 PMID: 14329143
  3. SPECIFIC COMPLEMENT-FIXING ANTIGENS IN POLYOMA TUMORS AND TRANSFORMED CELLS.
    Virology. 1965 Jan;25:55-61 PMID: 14277085
  4. DETECTION OF SPECIFIC ANTIGEN IN SV40-TRANSFORMED CELLS BY IMMUNOFLUORESCENCE.
    J Exp Med. 1964 Aug 1;120:121-8 PMID: 14206435
  5. A SPECIFIC COMPLEMENT-FIXING ANTIGEN PRESENT IN SV40 TUMOR AND TRANSFORMED CELLS.
    Proc Natl Acad Sci U S A. 1963 Dec;50:1148-56 PMID: 14096190
  6. Studies on cells rendered neoplastic by polyoma virus: the problem of the presence of virus-related materials.
    Virology. 1962 Jan;16:41-51 PMID: 13926482
  7. Studies of mouse polyoma virus infection. 1. Procedures for quantitation and detection of virus.
    J Exp Med. 1959 Apr 1;109(4):379-91 PMID: 13641563
  8. Localization of gene functions in polyoma virus DNA.
    Proc Natl Acad Sci U S A. 1976 Nov;73(11):4169-73 PMID: 186787
  9. Biological activity of polyoma viral DNA in mice and hamsters.
    J Virol. 1979 Mar;29(3):990-6 PMID: 221686
  10. Multiple forms of polyoma virus tumor antigens from infected and transformed cells.
    J Virol. 1979 Mar;29(3):881-7 PMID: 221679
  11. Characterization of T antigens in polyoma-infected and transformed cells.
    Cell. 1978 Sep;15(1):65-77 PMID: 212199
  12. Conditions leading to the establishment of the N (a gene dependent) and A (a gene independent) transformed states after polyoma virus infection of rat fibroblasts.
    J Virol. 1978 Nov;28(2):421-6 PMID: 214570
  13. Cellular and cell-free synthesis of simian virus 40 T-antigens in permissive and transformed cells.
    J Virol. 1978 Feb;25(2):587-95 PMID: 203721
  14. Polyoma virus defective DNAs. I. Physical maps of a related set of defective molecules (D76, D91, D92).
    J Mol Biol. 1977 Dec 5;117(2):473-95 PMID: 203698
  15. Temperature-sensitive growth regulation in one type of transformed rat cells induced by the tsa mutant of polyoma virus.
    J Virol. 1977 Dec;24(3):721-8 PMID: 201774
  16. Properties of simian virus 40 and BK virus tumor antigens form productively infected and transformed cells.
    Virology. 1978 Mar;85(1):137-45 PMID: 205997
  17. Relationship between the methionine tryptic peptides of simian virus 40 and BK virus tumor antigens.
    J Virol. 1977 Oct;24(1):319-25 PMID: 198582
  18. Hr-t and ts-a: two early gene functions of polyoma virus.
    Virology. 1977 Apr;77(2):610-24 PMID: 193256
  19. Complementation between temperature-sensitive (ts) and host range nontransforming (hr-t) mutants of polyoma virus.
    Virology. 1977 Apr;77(2):589-97 PMID: 193254
  20. Cell-free translation of simian virus 40 early messenger RNA coding for viral T-antigen.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):457-61 PMID: 191809
  21. Polyoma DNA: a physical map.
    Proc Natl Acad Sci U S A. 1974 May;71(5):2077-81 PMID: 4365585
  22. Polyoma gene function required for viral DNA synthesis.
    Virology. 1973 Sep;55(1):127-35 PMID: 4353950
  23. Complementation and transformation by temperature-sensitive mutants of polyoma virus.
    Virology. 1969 May;38(1):120-5 PMID: 4306521
  24. Selective extraction of polyoma DNA from infected mouse cell cultures.
    J Mol Biol. 1967 Jun 14;26(2):365-9 PMID: 4291934
  25. Heat-labile serum factor required for immunofluorescence of polyoma tumor antigens.
    Science. 1966 Sep 2;153(3740):1122-3 PMID: 4288238
  26. Host range mutants of polyoma virus.
    Proc Natl Acad Sci U S A. 1970 Sep;67(1):394-9 PMID: 4318787
  27. Virus-specific RNA in cells productively infected or transformed by polyoma virus.
    Cold Spring Harb Symp Quant Biol. 1975;39 Pt 1:187-98 PMID: 169059
  28. Construction of the genetic map of the polyoma genome.
    J Virol. 1976 Jun;18(3):824-32 PMID: 178924
  29. Altered patterns of protein synthesis in infection by SV40 mutants.
    Cold Spring Harb Symp Quant Biol. 1975;39 Pt 1:9-15 PMID: 169100
  30. Studies of polyoma virus DNA: cleavage map of the polyoma virus genome.
    J Virol. 1975 Jan;15(1):191-8 PMID: 163343
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.76.8.3713
Published
1979-08-00
Pages
3713-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC383903
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.76.8.3713 PMC Full Text View journal details
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