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PMID: 226986 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Reactivation of silent rRNA genes by simian virus 40 in human-mouse hybrid cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 8 ·1979-08-00 ·Pages 3885-9

Soprano KJ, Dev VG, Croce CM, Baserga R

Abstract

Mouse-human hybrid cells were used to study the ability of simian virus 40 to regulate the expression of rRNA genes in vivo. In these hybrid cells, only the rRNA genes of the dominant species are expressed; the genes for the rRNA of the recessive species are silent. Simian virus 40 infection of these hybrids led to the production of two distinct 28S rRNA species as analyzed by agarose/2.4% polyacrylamide gel electrophoresis. These species were identified as human and mouse rRNAs. This result was confirmed by histochemical studies which indicated that the nucleolus organizer regions of both mouse and human chromosomes were actively synthesizing rRNA in the virus-infected hybrid cells. These results indicate that simian virus 40 infection can induce the expression of otherwise silent rRNA genes.

MeSH Terms
Animals Humans Hybrid Cells/physiology Mice RNA, Ribosomal/genetics Simian virus 40/genetics Species Specificity Time Factors Tumor Virus Infections/genetics
Chemicals
RNA, Ribosomal
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Soprano K J
Dev V G
Croce C M
Baserga R
References (26)
26 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.76.8.3885
Published
1979-08-00
Pages
3885-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC383940
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.76.8.3885 PMC Full Text View journal details
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