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PMID: 227834 Published · ppublish English Journal Article

Deoxyribonucleic acid and outer membrane: binding to outer membrane involves a specific protein.

Journal of bacteriology ·Vol. 140 ·No. 1 ·1979-10-00 ·Pages 43-9

Wolf-Watz H, Norqvist A

Abstract

The binding of deoxyribonucleic acid (DNA) to the outer membrane of Escherichia coli was examined. The amount of DNA found to be bound to outer membrane was low and was estimated to be about 0.4% of the total DNA. Treatment of cells with chloramphenicol or rifampin caused a disassociation of the apparent DNA-outer membrane complex. The results presented here suggest that the binding between membrane and DNA is specific and involves a membrane protein having a molecular weight of 13,000.

MeSH Terms
Bacterial Proteins/metabolism Cell Membrane/metabolism Chloramphenicol/pharmacology DNA Helicases/metabolism DNA, Bacterial/metabolism Escherichia coli/metabolism Membrane Proteins/metabolism Molecular Weight Rifampin/pharmacology
Chemicals
Bacterial Proteins DNA, Bacterial Membrane Proteins Chloramphenicol DNA Helicases Rifampin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wolf-Watz H
Norqvist A
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25 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.140.1.43-49.1979
Published
1979-10-00
Pages
43-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC216777
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.140.1.43-49.1979 PMC Full Text View journal details
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