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PMID: 22840402 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

The lncRNA Malat1 is dispensable for mouse development but its transcription plays a cis-regulatory role in the adult.

Cell reports ·Vol. 2 ·No. 1 ·2012-07-26 ·Pages 111-23

Zhang B, Arun G, Mao YS, Lazar Z, Hung G, Bhattacharjee G, Xiao X, Booth CJ, Wu J, Zhang C, Spector DL

Abstract

Genome-wide studies have identified thousands of long noncoding RNAs (lncRNAs) lacking protein-coding capacity. However, most lncRNAs are expressed at a very low level, and in most cases there is no genetic evidence to support their in vivo function. Malat1 (metastasis associated lung adenocarcinoma transcript 1) is among the most abundant and highly conserved lncRNAs, and it exhibits an uncommon 3'-end processing mechanism. In addition, its specific nuclear localization, developmental regulation, and dysregulation in cancer are suggestive of it having a critical biological function. We have characterized a Malat1 loss-of-function genetic model that indicates that Malat1 is not essential for mouse pre- and postnatal development. Furthermore, depletion of Malat1 does not affect global gene expression, splicing factor level and phosphorylation status, or alternative pre-mRNA splicing. However, among a small number of genes that were dysregulated in adult Malat1 knockout mice, many were Malat1 neighboring genes, thus indicating a potential cis-regulatory role of Malat1 gene transcription.

MeSH Terms
Age Factors Aging/genetics,metabolism Animals Female Gene Expression Regulation, Developmental Growth and Development/genetics Male Mice Mice, Inbred BALB C Mice, Knockout Models, Biological RNA, Long Noncoding/genetics,metabolism,physiology Regulatory Sequences, Ribonucleic Acid/genetics,physiology Transcription, Genetic/genetics
Chemicals
Malat1 long non-coding RNA, mouse RNA, Long Noncoding Regulatory Sequences, Ribonucleic Acid
Authors & Affiliations
11 authors, click to expand affiliations / ORCID
Zhang Bin
Cold Spring Harbor Laboratory, One Bungtown Road, Cold Spring Harbor, NY 11724, USA.
Arun Gayatri
Mao Yuntao S
Lazar Zsolt
Hung Gene
Bhattacharjee Gourab
Xiao Xiaokun
Booth Carmen J
Wu Jie
Zhang Chaolin
Spector David L
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Article Info
Journal
Cell reports
Abbr.
Cell Rep
ISSN
2211-1247
DOI
10.1016/j.celrep.2012.06.003
Published
2012-07-26
Epub
2012-00-28
Pages
111-23
Language
English
Region
United States
NLM ID
101573691
PMCID
PMC3408587
Subset
IM
Grants
PHS HHS · 42694 · United States
NIGMS NIH HHS · K99GM95713 · United States
NCI NIH HHS · 5P01CA013106-40 · United States
NIGMS NIH HHS · K99 GM095713 · United States
PHS HHS · W81XWH-10-1-0190 · United States
NCI NIH HHS · P30 CA045508 · United States
NCI NIH HHS · P01 CA013106 · United States
NIGMS NIH HHS · R01 GM042694 · United States
NCI NIH HHS · 2P30CA45508-24 · United States
External Links
PubMed source DOI: 10.1016/j.celrep.2012.06.003 PMC Full Text View journal details
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