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PMID: 22909819 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A new class of carriers that transport selective cargo from the trans Golgi network to the cell surface.

The EMBO journal ·Vol. 31 ·No. 20 ·2012-10-17 ·Pages 3976-90

Wakana Y, van Galen J, Meissner F, Scarpa M, Polishchuk RS, Mann M, Malhotra V

Abstract

We have isolated a membrane fraction enriched in a class of transport carriers that form at the trans Golgi network (TGN) and are destined for the cell surface in HeLa cells. Protein kinase D (PKD) is required for the biogenesis of these carriers that contain myosin II, Rab6a, Rab8a, and synaptotagmin II, as well as a number of secretory and plasma membrane-specific cargoes. Our findings reveal a requirement for myosin II in the migration of these transport carriers but not in their biogenesis per se. Based on the cargo secreted by these carriers we have named them CARTS for CARriers of the TGN to the cell Surface. Surprisingly, CARTS are distinct from the carriers that transport vesicular stomatitis virus (VSV)-G protein and collagen I from the TGN to the cell surface. Altogether, the identification of CARTS provides a valuable means to understand TGN to cell surface traffic.

MeSH Terms
Biological Transport/physiology Cell Membrane/metabolism HeLa Cells Humans Intercellular Signaling Peptides and Proteins Lectins/metabolism Membrane Glycoproteins/metabolism Membrane Proteins/metabolism Myosin Type II/physiology Protein Kinase C/metabolism Synaptotagmin II/metabolism Transport Vesicles/classification,physiology,ultrastructure rab GTP-Binding Proteins/metabolism trans-Golgi Network/metabolism
Chemicals
Intercellular Signaling Peptides and Proteins Lectins Membrane Glycoproteins Membrane Proteins Rab6 protein Synaptotagmin II TGOLN2 protein, human ZG16B protein, human protein kinase D Protein Kinase C Myosin Type II RAB8A protein, human rab GTP-Binding Proteins
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Wakana Yuichi
Cell and Developmental Biology Programme, Centre for Genomic Regulation (CRG) and UPF, Barcelona, Spain.
van Galen Josse
Meissner Felix
Scarpa Margherita
Polishchuk Roman S
Mann Matthias
Malhotra Vivek
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
1460-2075
DOI
10.1038/emboj.2012.235
Published
2012-10-17
Epub
2012-00-21
Pages
3976-90
Language
English
Region
England
NLM ID
8208664
PMCID
PMC3474920
Subset
IM
Grants
Telethon · GTF08001 · Italy
Corrections
CommentIn
PMID: 22940689
External Links
PubMed source DOI: 10.1038/emboj.2012.235 PMC Full Text View journal details
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