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PMID: 229240 Published · ppublish English Journal Article

The genome of infectious bursal disease virus consists of two segments of double-stranded RNA.

Journal of virology ·Vol. 31 ·No. 3 ·1979-09-00 ·Pages 584-9

Müller H, Scholtissek C, Becht H

Abstract

The RNA of infectious bursal disease virus was reexamined in a detailed analysis. It could be established that its genome consists of two segments of double-stranded RNA. The RNA is RNase resistant and has a sedimentation coefficient of 14S and a buoyant density of 1.62 g/ml. The purine/pyrimidine ratio is nearly 1; the guanine plus cytosine content is 55.3%; the Tm is 95.5 degrees C. The molecular weights of the two double-stranded segments were determined to be 2.2 x 10(6) and 2.5 x 10(6).

MeSH Terms
Centrifugation, Density Gradient Infectious bursal disease virus/analysis Molecular Weight Nucleotides/analysis RNA, Double-Stranded/analysis RNA, Viral/analysis Reoviridae/analysis Ribonucleases/metabolism
Chemicals
Nucleotides RNA, Double-Stranded RNA, Viral Ribonucleases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Müller H
Scholtissek C
Becht H
References (16)
16 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
Published
1979-09-00
Pages
584-9
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC353486
Subset
IM
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