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PMID: 22936217 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Primary culture and immortalization of human fallopian tube secretory epithelial cells.

Nature protocols ·Vol. 7 ·No. 9 ·2012-09-00 ·Pages 1755-64

Karst AM, Drapkin R

Abstract

Primary human fallopian tube secretory epithelial cell (FTSEC) cultures are useful for studying normal fallopian tube epithelial biology, as well as for developing models of fallopian tube disease, such as cancer. Because of the limited ability of primary human FTSECs to proliferate in vitro, it is necessary to immortalize them in order to establish a cell line that is suitable for long-term culture and large-scale in vitro experimentation. This protocol describes the isolation of FTSECs from human fallopian tube tissue, conditions for primary FTSEC culture and techniques for establishing immortal FTSEC lines. The entire process, from primary cell isolation to establishment of an immortal cell line, may take up to 2 months. Once established, immortal FTSECs can typically be maintained for at least 30 passages.

MeSH Terms
Cell Culture Techniques/methods Cell Line, Transformed Cell Transformation, Neoplastic Epithelial Cells/cytology Fallopian Tubes/cytology Female Humans
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Karst Alison M
Department of Medical Oncology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts, USA.
Drapkin Ronny
References (28)
28 references, click to expand
  1. Molecular requirements for transformation of fallopian tube epithelial cells into serous carcinoma.
    Neoplasia. 2011 Oct;13(10):899-911 PMID: 22028616
  2. The new face of ovarian cancer modeling: better prospects for detection and treatment.
    F1000 Med Rep. 2011;3:22 PMID: 22076125
  3. A simple and quick method to concentrate MSCV retrovirus.
    Blood Cells Mol Dis. 2004 Jul-Aug;33(1):64-7 PMID: 15223013
  4. Validating genetic risk associations for ovarian cancer through the international Ovarian Cancer Association Consortium.
    Br J Cancer. 2009 Jan 27;100(2):412-20 PMID: 19127255
  5. Gene transfer by retrovirus vectors occurs only in cells that are actively replicating at the time of infection.
    Mol Cell Biol. 1990 Aug;10(8):4239-42 PMID: 2370865
  6. Intercepting pelvic cancer in the distal fallopian tube: theories and realities.
    Mol Oncol. 2009 Apr;3(2):165-70 PMID: 19383378
  7. Common variants at 19p13 are associated with susceptibility to ovarian cancer.
    Nat Genet. 2010 Oct;42(10):880-4 PMID: 20852633
  8. PAX8 reliably distinguishes ovarian serous tumors from malignant mesothelioma.
    Am J Surg Pathol. 2010 May;34(5):627-35 PMID: 20414098
  9. A candidate precursor to serous carcinoma that originates in the distal fallopian tube.
    J Pathol. 2007 Jan;211(1):26-35 PMID: 17117391
  10. Nonradioactive detection of telomerase activity using the telomeric repeat amplification protocol.
    Nat Protoc. 2006;1(3):1583-90 PMID: 17406450
  11. Precursors to pelvic serous carcinoma and their clinical implications.
    Gynecol Oncol. 2009 Jun;113(3):391-6 PMID: 19237187
  12. Common alleles in candidate susceptibility genes associated with risk and development of epithelial ovarian cancer.
    Int J Cancer. 2011 May 1;128(9):2063-74 PMID: 20635389
  13. Does bilateral salpingectomy with ovarian retention warrant consideration as a temporary bridge to risk-reducing bilateral oophorectomy in BRCA1/2 mutation carriers?
    Am J Obstet Gynecol. 2011 Jan;204(1):19.e1-6 PMID: 20619389
  14. Integrated genomic analyses of ovarian carcinoma.
    Nature. 2011 Jun 29;474(7353):609-15 PMID: 21720365
  15. The fallopian tube: primary site of most pelvic high-grade serous carcinomas.
    Int J Gynecol Cancer. 2009 Jan;19(1):58-64 PMID: 19258943
  16. Emerging roles for PAX8 in ovarian cancer and endosalpingeal development.
    Gynecol Oncol. 2007 Feb;104(2):331-7 PMID: 17064757
  17. The tubal fimbria is a preferred site for early adenocarcinoma in women with familial ovarian cancer syndrome.
    Am J Surg Pathol. 2006 Feb;30(2):230-6 PMID: 16434898
  18. Ovarian cancer pathogenesis: a model in evolution.
    J Oncol. 2010;2010:932371 PMID: 19746182
  19. Role of genetic polymorphisms and ovarian cancer susceptibility.
    Mol Oncol. 2009 Apr;3(2):171-81 PMID: 19383379
  20. Molecular pathogenesis and extraovarian origin of epithelial ovarian cancer--shifting the paradigm.
    Hum Pathol. 2011 Jul;42(7):918-31 PMID: 21683865
  21. Modeling high-grade serous ovarian carcinogenesis from the fallopian tube.
    Proc Natl Acad Sci U S A. 2011 May 3;108(18):7547-52 PMID: 21502498
  22. New insights into the pathogenesis of serous ovarian cancer and its clinical impact.
    J Clin Oncol. 2008 Nov 10;26(32):5284-93 PMID: 18854563
  23. Are all pelvic (nonuterine) serous carcinomas of tubal origin?
    Am J Surg Pathol. 2010 Oct;34(10):1407-16 PMID: 20861711
  24. Ex vivo culture of primary human fallopian tube epithelial cells.
    J Vis Exp. 2011 May 09;(51): PMID: 21610668
  25. A genome-wide association study identifies susceptibility loci for ovarian cancer at 2q31 and 8q24.
    Nat Genet. 2010 Oct;42(10):874-9 PMID: 20852632
  26. Integrated genome-wide DNA copy number and expression analysis identifies distinct mechanisms of primary chemoresistance in ovarian carcinomas.
    Clin Cancer Res. 2009 Feb 15;15(4):1417-27 PMID: 19193619
  27. The distal fallopian tube: a new model for pelvic serous carcinogenesis.
    Curr Opin Obstet Gynecol. 2007 Feb;19(1):3-9 PMID: 17218844
  28. Primary ex vivo cultures of human fallopian tube epithelium as a model for serous ovarian carcinogenesis.
    Oncogene. 2010 Feb 25;29(8):1103-13 PMID: 19935705
Article Info
Journal
Nature protocols
Abbr.
Nat Protoc
ISSN
1750-2799
DOI
10.1038/nprot.2012.097
Published
2012-09-00
Epub
2012-00-30
Pages
1755-64
Language
English
Region
England
NLM ID
101284307
PMCID
PMC7433321
Subset
IM
Grants
NCI NIH HHS · P50 CA105009 · United States
CIHR · Canada
External Links
PubMed source DOI: 10.1038/nprot.2012.097 PMC Full Text View journal details
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