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PMID: 2296589 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Translation of bicistronic viral mRNA in transfected cells: regulation at the level of elongation.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 1 ·1990-01-00 ·Pages 328-32

Fajardo JE, Shatkin AJ

Abstract

The S1 species of mammalian reovirus mRNA, like a number of other viral but not cellular mRNAs, codes for two dissimilar polypeptides by initiation of translation at two 5'-proximal, out-of-frame AUG codons. To determine if uninfected cells can utilize bicistronic genes, a bovine papilloma virus-based vector system was used to select mouse C127 cell lines containing multiple integrated copies of the reovirus S1 gene. These cell lines produced both reovirus polypeptides from a single mRNA. In addition, studies of COS cells transfected with the S1 gene containing small changes around the first AUG suggest that bicistronic mRNA translation is regulated at the level of elongation. A model is proposed in which ribosomes engaged in translation of one reading frame interfere with movement of ribosomes in the other frame because of differences in codon usage. Expression of bicistronic genes may be similarly regulated in virus-infected cells.

MeSH Terms
Animals Base Sequence Cadmium/pharmacology Cadmium Chloride Cell Line DNA, Viral/genetics Gene Expression Regulation, Viral Genes Genetic Vectors Mice Molecular Sequence Data Peptide Chain Elongation, Translational Plasmids Protein Biosynthesis/drug effects RNA, Messenger/genetics RNA, Viral/genetics Reoviridae/genetics Transfection Viral Proteins/biosynthesis,genetics
Chemicals
DNA, Viral RNA, Messenger RNA, Viral Viral Proteins Cadmium Cadmium Chloride
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Fajardo J E
Center for Advanced Biotechnology and Medicine, Piscataway, NJ 08854-5638.
Shatkin A J
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.87.1.328
Published
1990-01-00
Pages
328-32
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC53256
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.87.1.328 PMC Full Text View journal details
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