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PMID: 2298482 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Adherence of Staphylococcus epidermidis to fibrin-platelet clots in vitro mediated by lipoteichoic acid.

Infection and immunity ·Vol. 58 ·No. 2 ·1990-02-00 ·Pages 315-9

Chugh TD, Burns GJ, Shuhaiber HJ, Bahr GM

Abstract

The adherence of two strains of Staphylococcus epidermidis to human fibrin-platelet clots in vitro was investigated. Both strains were noncapsulated, nonhemagglutinating, and nonslime producers. Binding was not related to surface charge, carbohydrate profile, or hydrophobicity of the bacteria. Adherence was reduced four- to sixfold (P less than 0.001) on pretreatment of bacteria with lipase, while neuraminidase, trypsin, phospholipase C, and sodium periodate did not alter their binding. Pretreatment of bacteria with substances known to bind lipoteichoic acid (LTA), such as human albumin and anti-LTA antibodies, also resulted in a fourfold (P less than 0.001) reduction in adherence. Prior incubation of clots with free LTA, but not with deacylated LTA, produced a fourfold (P less than 0.001) decrease in the adherence of homologous and heterologous strains of S. epidermidis. A similar reduction was also observed when LTAs derived from Staphylococcus aureus and Streptococcus pyogenes were used. These data provide evidence that the lipid moiety of LTA has a central role in the adherence of S. epidermidis to fibrin-platelet clots in vitro.

MeSH Terms

Bacterial Adhesion Blood Platelets/microbiology,physiology Fibrin/metabolism Humans Lipopolysaccharides/pharmacology Staphylococcus epidermidis/physiology Teichoic Acids/pharmacology

Chemicals

Lipopolysaccharides Teichoic Acids lipoteichoic acid Fibrin

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Chugh T D

Department of Microbiology, Faculty of Medicine, University of Kuwait.

Burns G J

Shuhaiber H J

Bahr G M

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24 references, click to expand

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Article Info

Journal

Infection and immunity

Abbr.

Infect Immun

ISSN

0019-9567

DOI

10.1128/iai.58.2.315-319.1990

Published

1990-02-00

Pages

315-9

Language

English

Region

United States

NLM ID

0246127

PMCID

PMC258457

Subset

External Links

PubMed source DOI: 10.1128/iai.58.2.315-319.1990 PMC Full Text View journal details

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Adherence of Staphylococcus epidermidis to fibrin-platelet clots in vitro mediated by lipoteichoic acid.

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