The author carried out studies on 600 Bulgarians from the south Bulgaria (the town of Plovdiv) and 232 gypsies. There was gene frequency of AK1=0.9633 and AK2=0.0367 in the Bulgariana and respectively AK1=0.9784 and AK2=0.0216 in the gypsies as the differences between both populations were insignificant. All data, published up to now, were summarised connected with the examination of the Bulgarians for AK (1278) and the estimated mean values for gene frequencies were: AK1=0.9648 and =K2=0.0352). Gene frequencies of the Bulgarians, compared with other populations, revealed that they were in the ranges of the mean European values for gene frequencies and differed substantially from those of the nergos and some Asian nations (Japanese, Chinese). The theoretical probability for excluding fatherhood by means of the system of Ak for the Bulgarian was 3.28%.
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