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PMID: 2302195 Published · ppublish English Journal Article

Electrofusion of oriented Schizosaccharomyces pombe cells through apical protoplast-protuberances.

Biochemical and biophysical research communications ·Vol. 166 ·No. 1 ·1990-01-15 ·Pages 113-8

Vondrejs V, Pavlícek I, Kothera M, Palková Z

Abstract

The electrofusion of oriented Schizosaccharomyces pombe cells through apical protoplast-protuberances was demonstrated. The protuberances arose after an exposure of early-exponential phase cells to digestive enzymes from hepatopancreas of Helix pomatia. The orientation of cylindric cells within pearl chains was produced by the application of inhomogenous alternating electric fields.

MeSH Terms
Electric Stimulation Membrane Fusion Protoplasts/drug effects,physiology,ultrastructure Saccharomycetales/physiology Schizosaccharomyces/physiology Sorbitol/pharmacology
Chemicals
Sorbitol
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Vondrejs V
Department of Biotechnology, Faculty of Science, Charles University, Prague, Czechoslovakia.
Pavlícek I
Kothera M
Palková Z
Article Info
Journal
Biochemical and biophysical research communications
Abbr.
Biochem Biophys Res Commun
ISSN
0006-291X
Published
1990-01-15
Pages
113-8
Language
English
Region
United States
NLM ID
0372516
Subset
IM
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