Home LiteratureArticle Details
PMID: 2304140 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Isolation and partial characterization of an unusual human immunodeficiency retrovirus from two persons of west-central African origin.

Journal of virology ·Vol. 64 ·No. 3 ·1990-03-00 ·Pages 1207-16

De Leys R, Vanderborght B, Vanden Haesevelde M, Heyndrickx L, van Geel A, Wauters C, Bernaerts R, Saman E, Nijs P, Willems B

Abstract

An unusual human retrovirus was isolated from two patients with persistent generalized lymphadenopathy who originate from West-Central Africa and are currently residing in Belgium. Although the virus shared a number of the same biological and morphological properties as human immunodeficiency retrovirus type 1 (HIV-1) and HIV-2, significant antigenic differences could be demonstrated. Several of the viral proteins also differed in molecular weight from the corresponding HIV-1 and HIV-2 proteins. Partial chemical cleavage of the most highly conserved viral proteins resulted in patterns which differed from those of HIV-1 and HIV-2. Furthermore, nucleic acid hybridization experiments were capable of discriminating between the virus types. Sequence analysis of the viral U3 region revealed a unique enhancer organization not found in other immunodeficiency viruses. The data indicated that the new isolate is more closely related to HIV-1 than to HIV-2 but clearly differs in a number of important respects.

MeSH Terms
Africa, Central Africa, Western Antibodies, Monoclonal Antigen-Antibody Complex Antigens, Viral/analysis Base Sequence Cloning, Molecular HIV/classification,genetics,isolation & purification HIV-1/classification HIV-2/classification Humans Immunoblotting Molecular Sequence Data Nucleic Acid Conformation Nucleic Acid Hybridization RNA, Viral/genetics Sequence Homology, Nucleic Acid Viral Proteins/analysis
Chemicals
Antibodies, Monoclonal Antigen-Antibody Complex Antigens, Viral RNA, Viral Viral Proteins
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
De Leys R
Innogenetics N.V., Antwerp, Belgium.
Vanderborght B
Vanden Haesevelde M
Heyndrickx L
van Geel A
Wauters C
Bernaerts R
Saman E
Nijs P
Willems B
References (38)
38 references, click to expand
  1. Isolation of a T-lymphotropic retrovirus from a patient at risk for acquired immune deficiency syndrome (AIDS).
    Science. 1983 May 20;220(4599):868-71 PMID: 6189183
  2. Regulation of interleukin 2 receptor alpha subunit (Tac or CD25 antigen) gene expression: binding of inducible nuclear proteins to discrete promoter sequences correlates with transcriptional activation.
    Proc Natl Acad Sci U S A. 1988 Jun;85(12):4468-72 PMID: 3132714
  3. Frequent detection and isolation of cytopathic retroviruses (HTLV-III) from patients with AIDS and at risk for AIDS.
    Science. 1984 May 4;224(4648):500-3 PMID: 6200936
  4. Antibodies reactive with human T-lymphotropic retroviruses (HTLV-III) in the serum of patients with AIDS.
    Science. 1984 May 4;224(4648):506-8 PMID: 6324345
  5. Isolation of lymphocytopathic retroviruses from San Francisco patients with AIDS.
    Science. 1984 Aug 24;225(4664):840-2 PMID: 6206563
  6. Molecular characterization of human T-cell leukemia (lymphotropic) virus type III in the acquired immune deficiency syndrome.
    Science. 1984 Dec 7;226(4679):1165-71 PMID: 6095449
  7. Characterization of long terminal repeat sequences of HTLV-III.
    Science. 1985 Feb 1;227(4686):538-40 PMID: 2981438
  8. Identification of a putative regulator of early T cell activation genes.
    Science. 1988 Jul 8;241(4862):202-5 PMID: 3260404
  9. Purification of the human immunodeficiency virus type 1 enhancer and TAR binding proteins EBP-1 and UBP-1.
    EMBO J. 1988 Jul;7(7):2117-30 PMID: 3138113
  10. Isolation and characterization of simian immunodeficiency virus from mandrills in Africa and its relationship to other human and simian immunodeficiency viruses.
    J Virol. 1988 Nov;62(11):4044-50 PMID: 3172337
  11. Repeated B motifs in the human immunodeficiency virus type I long terminal repeat enhancer region do not exhibit cooperative factor binding.
    Proc Natl Acad Sci U S A. 1988 Dec;85(24):9406-10 PMID: 3200827
  12. Signaling through T lymphocyte surface proteins, TCR/CD3 and CD28, activates the HIV-1 long terminal repeat.
    J Immunol. 1989 Jan 15;142(2):702-7 PMID: 2536062
  13. Isolation of human immunodeficiency virus type 2 from a homosexual man in Belgium.
    Eur J Clin Microbiol Infect Dis. 1988 Dec;7(6):816-8 PMID: 3145873
  14. Human immunodeficiency virus type 1 LTR TATA and TAR region sequences required for transcriptional regulation.
    EMBO J. 1989 Mar;8(3):765-78 PMID: 2721501
  15. HIV-2, the West African AIDS virus.
    AIDS. 1987 Sep;1(3):135-40 PMID: 3126752
  16. Nucleotide sequence of the AIDS virus, LAV.
    Cell. 1985 Jan;40(1):9-17 PMID: 2981635
  17. Isolation of T-cell tropic HTLV-III-like retrovirus from macaques.
    Science. 1985 Jun 7;228(4704):1201-4 PMID: 3159089
  18. Genomic diversity of human T-lymphotropic virus type III (HTLV-III).
    Science. 1985 Aug 23;229(4715):759-62 PMID: 2992084
  19. Selective cytotoxicity of AIDS virus infection towards HTLV-I-transformed cell lines.
    Int J Cancer. 1985 Oct 15;36(4):445-51 PMID: 2995259
  20. Recovery of AIDS-associated retroviruses from patients with AIDS or AIDS-related conditions and from clinically healthy individuals.
    J Infect Dis. 1985 Oct;152(4):734-8 PMID: 2413137
  21. Genomic heterogeneity of AIDS retroviral isolates from North America and Zaire.
    Science. 1985 Nov 22;230(4728):949-51 PMID: 2997922
  22. Serological evidence for virus related to simian T-lymphotropic retrovirus III in residents of west Africa.
    Lancet. 1985 Dec 21-28;2(8469-70):1387-9 PMID: 2867393
  23. Differential susceptibility to the acquired immunodeficiency syndrome retrovirus in cloned cells of human leukemic T-cell line Molt-4.
    J Virol. 1986 Mar;57(3):1159-62 PMID: 2419583
  24. Virus related to but not identical with LAV/HTLV-III in Cameroon.
    Lancet. 1986 Apr 12;1(8485):854 PMID: 2870334
  25. Genomic variability of selected LAV-related AIDS retroviruses.
    AIDS Res. 1986 Feb;2(1):19-30 PMID: 2424466
  26. Genetic variability of the AIDS virus: nucleotide sequence analysis of two isolates from African patients.
    Cell. 1986 Jul 4;46(1):63-74 PMID: 2424612
  27. Isolation of a new human retrovirus from West African patients with AIDS.
    Science. 1986 Jul 18;233(4761):343-6 PMID: 2425430
  28. Regulation of human interleukin-2 gene: functional DNA sequences in the 5' flanking region for the gene expression in activated T lymphocytes.
    Cell. 1986 Aug 1;46(3):401-5 PMID: 3015413
  29. Effects of the modification of transfer buffer composition and the renaturation of proteins in gels on the recognition of proteins on Western blots by monoclonal antibodies.
    Anal Biochem. 1986 Aug 15;157(1):144-53 PMID: 3532863
  30. AIDS in Africa: an epidemiologic paradigm.
    Science. 1986 Nov 21;234(4779):955-63 PMID: 3022379
  31. Cross-reactivity to human T-lymphotropic virus type III/lymphadenopathy-associated virus and molecular cloning of simian T-cell lymphotropic virus type III from African green monkeys.
    Proc Natl Acad Sci U S A. 1986 Dec;83(24):9754-8 PMID: 3491989
  32. Activation of the HIV-1 LTR by T cell mitogens and the trans-activator protein of HTLV-I.
    Science. 1987 Dec 11;238(4833):1575-8 PMID: 2825351
  33. Isolation of simian immunodeficiency virus from African green monkeys and seroepidemiologic survey of the virus in various non-human primates.
    Int J Cancer. 1988 Jan 15;41(1):115-22 PMID: 2447023
  34. Interactions of cellular proteins involved in the transcriptional regulation of the human immunodeficiency virus.
    EMBO J. 1987 Dec 1;6(12):3761-70 PMID: 3428273
  35. Comparison of simian immunodeficiency virus isolates.
    Nature. 1988 Feb 18;331(6157):619-22 PMID: 2893293
  36. HIV infection in Nigeria.
    AIDS. 1988 Feb;2(1):61-2 PMID: 3128999
  37. Characterization of antigen receptor response elements within the interleukin-2 enhancer.
    Mol Cell Biol. 1988 Apr;8(4):1715-24 PMID: 3260003
  38. Detection, isolation, and continuous production of cytopathic retroviruses (HTLV-III) from patients with AIDS and pre-AIDS.
    Science. 1984 May 4;224(4648):497-500 PMID: 6200935
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.64.3.1207-1216.1990
Published
1990-03-00
Pages
1207-16
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC249235
Subset
IM
Databases
GENBANK
M31171
External Links
PubMed source DOI: 10.1128/JVI.64.3.1207-1216.1990 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2